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1、TranscriptionalRegulationinEukaryotesConcepts,Strategies,andTechniquesMichaelCareyStephenT.SmaleCOLDSPRINGHARBORLABORATORYPRESSTranscriptionalRegulationinEukaryotesConcepts,Strategies,andTechniquesMichaelCareyStephenT.SmaleUniversityofCalifornia,HowardHug
2、hesMedicalInstituteandLosAngelesUniversityofCalifornia,LosAngelesTranscriptionalRegulationinEukaryotesConcepts,Strategies,andTechniquesAllrightsreserved©2000ColdSpringHarborLaboratoryPress,ColdSpringHarbor,NewYorkPrintedintheUnitedStatesofAmericaDevelopme
3、ntalEditor:JudyCuddihyDesktopEditors:DannydeBruin,SusanSchaeferAssistantDevelopmentalEditor:BirgitWoelkerInteriorBookDesign:DeniseWeissProjectCoordinator:MarylizM.DickersonCoverDesign:TonyUrgoProductionEditor:PatriciaBarkerCoverartrenderedbyMichaelHaykins
4、onCoverillustration:ThecoverschematicallyillustratesthestructureoftheRNApolymeraseIItranscriptioncomplexemergingfromablackbox.ItisacompositeillustrationoftheTFIIA-TBP-TATA(Geigeretal.Science272:830–836[1996];Tanetal.Nature381:127–151[1996]),andTFIIB-TBP-T
5、ATA(Nikolovetal.Nature377:119–128[1995])crystalstructuresrenderedbyMichaelHaykinson(UCLA)usingtheMolecularGraphicsstructuremodelingcomputerprogramInsightII.LibraryofCongressCataloging-in-PublicationDataCarey,Michael(MichaelF.)Transcriptionalregulationineu
6、karyotes:concepts,strategies,andtechniques/MichaelCarey,StephenT.Smale.p.cm.Includesbibliographicalreferencesandindex.ISBN0-87969-537-4(cloth)--ISBN0-87969-635-4(pbk.)1.Genetictranscription--Regulation.2.Transcriptionfactors.3.Genetictranscription--Regula
7、tion--Research--Methodology.I.SmaleT.II.Title.QH450.2.C3751999572.8´845--dc2199-049636109876543Studentsandresearchersusingtheproceduresinthismanualdosoattheirownrisk.ColdSpringHarborLaboratorymakesnorepresentationsorwarrantieswithrespecttothematerialsetfo
8、rthinthismanualandhasnoliabilityinconnectionwiththeuseofthesematerials.Proceduresforthehumanetreatmentofanimalsmustbeobservedatalltimes.Checkwiththelocalanimalfacilityforguidelines.Certainexperimentalproceduresinthi
