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1、GeneralandComparativeEndocrinology176(2012)173–181ContentslistsavailableatSciVerseScienceDirectGeneralandComparativeEndocrinologyjournalhomepage:www.elsevier.com/locate/ygcenBasicfibroblastgrowthfactorsuppressesmeiosisandpromotesmitosisofovariangermcellsinembryonicc
2、hickensa,1a,b,1aaaa,⇑BinHe,JinxingLin,JieLi,YulingMi,WeidongZeng,CaiqiaoZhangaDepartmentofVeterinaryMedicine,CollegeofAnimalSciences,ZhejiangUniversity,Hangzhou310058,ChinabShanghaiLaboratoryAnimalResearchCenter,Shanghai201203,ChinaarticleinfoabstractArticlehistory:
3、Basicfibroblastgrowthfactor(bFGForFGF2)playsdiverserolesinregulatingcellproliferation,migrationReceived12November2011anddifferentiationduringembryodevelopment.Inthisstudy,theeffectofbFGFonovariangermcellRevised12January2012developmentwasinvestigatedintheembryonicchi
4、ckenbyinvitroandinvivoexperiments.ResultsAccepted13January2012showedthataremarkabledecreaseinbFGFexpressionintheovariancortexwasmanifestedduringmei-Availableonline28January2012osisprogression.Withovaryorganculture,werevealedthatmeiosiswasinitiatedafterretinoicacid(R
5、A)treatmentalonebutwasdecreasedaftercombinedbFGFtreatmentthatwasdetectedbyrealtimeRT-Keywords:PCR,fluorescenceimmunohistochemistryandGiemsastaining.Further,nosignificantdifferenceinBasicfibroblastgrowthfactormRNAexpressionofeitherRAmetabolism-relatedenzymes(Raldh2andC
6、yp26b1)orRAreceptorsMeiosisMitosiswasdisplayedafterbFGFchallenge.ThisresultsuggeststhatthesuppressionofbFGFonmeiosiswasGermcellunlikelythroughinhibitionofRAsignaling.Inaddition,asamitogen,bFGFadministrationincreasedgermChickencellproliferation(viaBrdUincorporation)i
7、nculturedorganorcellsinvitroandalsoindevelopingOvaryembryosinvivo.Incontrast,blockadeofbFGFactionbySU5402(anFGFR1antagonist)orinhibitionofproteinkinaseCsignalingshowedinhibitedeffectofbFGFonmitosis.Inconclusion,bFGFsuppressesRA-inducedentryofgermcellsintomeiosistoen
8、sureembryonicovariangermcellstomaintainatundif-ferentiatedstatusandaccelerategermcellproliferationbybindingwithFGFR1involvingPKCactivation
