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《A drosophila gustatory receptor required for the responses to sucrose (2)》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、ADrosophilagustatoryreceptorrequiredfortheresponsestosucrose,glucose,andmaltoseidentifiedbymRNAtaggingYuchenJiao,SeokJunMoon,andCraigMontell*DepartmentsofBiologicalChemistryandNeuroscience,CenterforSensoryBiology,JohnsHopkinsUniversitySchoolofMedicine,Baltimore,MD21205Ed
2、itedbyKathrynV.Anderson,SloanKetteringInstitute,NewYork,NY,andapprovedJuly20,2007(receivedforreviewMarch15,2007)InDrosophila,detectionoftastantsisthoughttobemediatedbyavoidanceandattractivecompounds,respectively(4,5).Atleastmembersofafamilyof68gustatoryreceptors(Grs).How
3、ever,onlynineadditionalGrsareexpressedinthelabellum,and,basedononereceptor,Gr5a,hasbeenassociatedwithasugar,andittransgenicreporters,allarecoexpressedinsubsetsoftheappearstobeactivatedspecificallybytrehalose.ItisunclearGr66a-positiveGRNs(4,5).Thus,asinmammals(3),itappears
4、whetherothersugarreceptorsareactivatedbysingleormultiplethatthemajorityofGrsparticipateintheperceptionofaversivesugars.Currently,noGrsareknowntocolocalizewithGr5a.Suchcompounds.GrswouldbecandidatesugarreceptorsbecauseGr5a-expressingInadditiontoGr5a,theremustbeadditionals
5、ugar-stimulatedcellsfunctionintheresponsestoattractivetastants.HereweuseGrsbecauseGr5ahasbeenreportedtobetunedspecificallytoan‘‘mRNAtagging’’approachtoidentifyGrRNAsthatarecoex-trehaloseandGr5amutantfliesdisplaynormalbehavioralandpressedwithGr5a.WefoundthatallsevenGrsmos
6、trelatedtoelectrophysiologyresponsestosucrose(26–28).Othersugarrecep-Gr5a(Gr64a-fandGr61a)wereexpressedinGr5a-expressingcells,torswouldappeartobecoexpressedwithGr5abecauseGr5acellswhereasnoneoftheotherGrsexaminedwereenrichedintheseGrrespondtomultiplesugarsinadditiontotre
7、halose(29).However,neurons(GRNs).WecharacterizedtheroleofoneGr5a-relateditisalsounclearwhetherothersugar-activatedGrsareactivatedbyreceptor,Gr64a,andfoundthatitwasrequiredforthebehavioralasingleligandasappearstobethecaseforGr5a.responsestoglucose,sucrose,andmaltose.Gr64a
8、wasrequiredforHereweusedarapidscreeningapproachtoidentifyotherGrsGRNfunctionbecauseactionpotentialsindu
