bacterial stress responses_2nd_2011

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1、BACTERIALSTRESSRESPONSESSecondEditionThispageintentionallyleftblankBACTERIALSTRESSRESPONSESSecondEditionEditedbyGiselaStorzEuniceKennedyShriverInstituteofChildHealthandHumanDevelopmentBethesda,MarylandandRegineHenggeFreieUniversitätBerlinBerlin,GermanyWashington,DCCopyright©2011ASMPressAmeric

2、anSocietyforMicrobiology1752NSt.,N.W.Washington,DC20036-2904LibraryofCongressCataloging-in-PublicationDataBacterialstressresponses/editedbyGiselaStorzandRegineHengge.—2nded.p.;cm.Includesbibliographicalreferencesandindex.ISBN-13:978-1-55581-621-6(hardcover:alk.paper)ISBN-10:1-55581-621-5(hard

3、cover:alk.paper)1.Microorganisms—Physiology.2.Stress(Physiology)3.Adaptation(Physiology)I.Storz,Gisela.II.Hengge,Regine.III.AmericanSocietyforMicrobiology.[DNLM:1.BacterialPhysiologicalPhenomena.2.Bacteria—genetics.3.Bacteriametabolism.4.EnvironmentalMicrobiology.QW52]QR97.A1B332011571.2'93—d

4、c22201003545210987654321AllRightsReservedPrintedintheUnitedStatesofAmericaAddresseditorialcorrespondencetoASMPress,1752NSt.,N.W.,Washington,DC20036-2904,USA.SendorderstoASMPress,P.O.Box605,Herndon,VA20172,USA.Phone:800-546-2416;703-661-1593Fax:703-661-1501E-mail:books@asmusa.orgOnline:http://

5、estore.asm.orgCoverimage:Thetwopanelsshowthesameareaoffalse-colorfluorescenceimagesofasinglemicroarrayspot-tedwith50-nucleotideoligomersspecificforallopenreadingframesintheE.coliK-12genomethatwashybrid-izedwithamixtureofCy3-/Cy5-labeledcDNAobtainedwithRNAextractedfromstrainW3110.Thecellswereg

6、rownindefinedrichmediumandharvestedforRNApreparationduringgrowth(atanOD578of1.0)at37°Cor5minafterashiftfrom37°Cto42°C(preparationslabeledwithCy3orCy5,respectively).ThesecellsweretreatedexactlyasthecellsusedbyRothvanBogelenandFredNeidhardtforthe“historic”proteomicanalysisofheat-shockedE.colice

7、llsshownonthecoverofthefirsteditionofBacterialStressResponsesin2000.Theupperpanelshowsfluorescentsignalsderivedfromnon-heat-shockedcells.Thelowerpanelshowsfluorescentsignalsderivedfromheat-shockedcells.Spotsrepresentingheat-shock-induciblegen