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1、FoodChemistry130(2012)121–126ContentslistsavailableatScienceDirectFoodChemistryjournalhomepage:www.elsevier.com/locate/foodchemStudiesonpurificationandthemolecularmechanismofanovelACEinhibitorypeptidefromwheyproteinhydrolysatea,b,⇑abcDaodongPan,Jinxua
2、nCao,HuiqingGuo,BoZhaoaFacultyofLifeScience&Biotechnology,NingboUniversity,Ningbo315211,ChinabFacultyofFoodScience,NanjingNormalUniversity,Nanjing210097,ChinacFacultyofChemistryandMaterialScience,NanjingNormalUniversity,Nanjing210097,Chinaarticleinfo
3、abstractArticlehistory:ThisstudysoughttopurifyandidentifyanovelangiotensinI-convertingenzyme(ACE)inhibitorypeptideReceived24January2011fromwheyproteinhydrolysedbytrypsin.Thepeptide’saminoacidsequence,aswellasthemolecularReceivedinrevisedform18April20
4、11mechanismoftheinteractionsbetweenthepeptideandtheACE,werealsostudied.Usingultrafiltration,Accepted6July2011thehydrolysatewasseparatedintothreefractions.Thefractionwithmolecularweightof<6kDahadtheAvailableonline12July2011greatestACEinhibitoryactivity
5、andwasfurtherseparatedbysizeexclusionchromatographyonSepha-dexG-25andG-10columns.Reverse-phasehighperformanceliquidchromatography(RP-HPLC)wasKeywords:usedtoseparatethemostactivefraction.TheaminoacidsequenceofthepeptidewiththegreatestWheyproteinhydrol
6、ysateACEinhibitorycharacteristicswasconfirmedasLeu–Leu(LL).Themolecularmechanisms,position,type,ACEinhibitorypeptidesPurificationandidentificationandenergyoftheLL/ACEinteractionwereinvestigatedbyusingflexiblemoleculedockingtechnology.MoleculemechanismÓ20
7、11ElsevierLtd.Allrightsreserved.1.IntroductionACEinhibitorypeptidesareinactivewithinthesequenceoftheparentprotein,butcanbereleasedbyenzymatichydrolysiswithAngiotensin-Iconvertingenzyme(ACE)isadipeptidehydrolaseenzymes.Selectingtheproperenzymetohydrol
8、ysetheproteinisthatcatalysesboththeformationofthepotentvasoconstrictor,akeyfactorinobtainingpeptidesthatexhibitgreaterlevelsofangiotensin-II,andthedeactivationofbradykinin,avasodilatorACEinhibitorybehaviour.Trypsinisapromisingenzymetobepeptide.Theref
