冠状病毒Coronavirusl论文-2014 Molecular Survey of RNA Viruses in Hungarian Bats_ Discovering Novel Astroviruses, Coronaviruses, and Caliciviruses.pdf

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1、VECTOR-BORNEANDZOONOTICDISEASESVolume14,Number12,2014ªMaryAnnLiebert,Inc.DOI:10.1089/vbz.2014.1637MolecularSurveyofRNAVirusesinHungarianBats:DiscoveringNovelAstroviruses,Coronaviruses,andCaliciviruses1,21,23,745Ga´borKemenesi,BiankaDallos,Tama´sGo¨rfo¨l,

2、Sa´ndorBoldogh,Pe´terEsto´k,21,21,21,21,2Korne´liaKurucz,AnnaKutas,FanniFo¨ldes,Miklo´sOldal,Vikto´riaNe´meth,671,2VitoMartella,Krisztia´nBa´nyai,andFerencJakabAbstractBackground:Bat-bornevirusesposeapotentialrisktohumanhealthandarethefocusofincreasingsc

3、ientificinterest.Tostartgaininginformationaboutbat-transmittedvirusesinHungary,wetestedmultiplebatspeciesforseveralvirusgroupsbetween2012and2013.MaterialsandMethods:FecalsampleswerecollectedfrombatsacrossHungary.Weperformedgroup-specificRT-PCRscreeningfora

4、stro-,calici-,corona-,lyssa-,othoreo-,paramyxo-,androtaviruses.Positivesampleswereselectedandsequencedforfurtherphylogeneticanalyses.Results:Atotalof447fecalsamples,representing24Europeanbatspeciesweretested.Novelstrainsofastroviruses,coronaviruses,andca

5、licivirusesweredetectedandanalyzedphylogenetically.Outofthe447testedsamples,40(9%)batswerepositiveforatleastonevirus.Bat-transmittedastroviruses(BtAstV)weredetectedineightspecieswitha6.93%detectionrate(95%confidenceinterval[CI]4.854,9.571).Coronaviruses(B

6、tCoV)weredetectedinsevenbatspecieswithadetectionrateof1.79%(95%CI0.849,3.348),whereasnovelcaliciviruses(BtCalV)weredetectedinthreebatspecieswithadetectionrateof0.67%(95%CI0.189,1.780).Phylogeneticanalysesrevealedagreatdiversityamongastrovirusstrains,wher

7、eastheHungarianBtCoVstrainsclusteredtogetherwithbothalpha-andbetacoronavirusstrainsfromotherEuropeancountries.OneofthemostintriguingfindingsofourinvestigationisthediscoveryofnovelBtCalVsinEurope.TheHungarianBtCalVdidnotclusterwithanyofthecalcivirusgenerai

8、dentifiedinthefamilysofar.Conclusions:WehavesuccessfullyconfirmedBtCoVsinnumerousbatspecies.Furthermore,wehavede-Vector-BorneandZoonoticDiseases2014.14:846-855.scribednewbatspeciesharboringBtAstVsinEuropeandfoundnewspeciesof