RNAi ；Molecular mechanism of RNAi；Mechanism of DNA methylation by RNAi inducing等

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1、RNAi毛自朝mzcleemao@gmail.com农学院生物技术系Tel:522773213114297551OutlineofthistalkingIntroducingofgenesilenceHistoryanddefinitionofRNAMolecularmechanismofRNAiMaincomponentsofRNAiBiologicalFunctionofRNAiMechanismofDNAmethylationbyRNAiinducingExampleTranslationregulationbyRNAiUnansweredquestionApplicationReg

2、ulateGeneexpressionlevelDNA(pre-transcription)geneamplificationTranscriptionallevel(promoter,TF.EnhancerReducer)PostTranscriptionalLevel(mRNA,tRNAandrRNAmaturationSpicingExton,CapandpolyA,RNAi)TranslationalandposttranslationallevelOthernamesofpost-transcriptionalgenesilencing(PTGS):–genesilencing–

3、RNAsilencing–RNAinterference–Incertainfungi:quellingRNAicanspreadthroughoutcertainorganisms(C.elegans,plants).WhyisRNAiimportant?MostwidelyheldviewisthatRNAievolvedtoprotectthegenomefromviruses(orotherinvadingDNAsorRNAs)Recently,verysmall(micro)RNAshavebeendiscoveredinseveraleukaryotesthatregulate

4、developmentallyotherlargeRNAsMaybeanewusefortheRNAimechanismbesidesdefenseRecentapplicationsofRNAiModulationofHIV-1replicationbyRNAinterference.Hannon(2002).Potentandspecificinhibitionofhumanimmunodeficiencyvirustype1replicationbyRNAinterference.Anetal.(1999)Selectivesilencingofviralgeneexpression

5、inHPV-positivehumancervical（颈）carcinomacellstreatedwithsiRNA,aprimerofRNAinterference.Jungetal.2002.RNAinterferenceinadultmice.Mccaffreyetal.2002SuccessfulinactivationofendogenousOct-3/4andc-mosgenesinmousepreimplantationembryosandoocytesusingshortinterferingRNAs.LeBonetal.2002Posttranscriptionalg

6、enesilencingPromotersactiveGenehypermethylatedincodingregionPurpose-Viralimmunity?S.Grant(1999)Transcriptionalgenesilencing(TGS)Posttranscriptionalgenesilencing(PTGS)Thishasrecentlybeentermed“RNAi”PromoterssilencedGeneshypermethylatedinpromoterregionPurpose-Viralimmunity?ShorthistoryofRNAiDefiniti

7、on:theabilityofexogenousdouble-strandedRNA(dsRNA)tosuppresstheexpressionofthegenewhichcorrespondstothedsRNAsequence.1990:Introductionoftransgeneshomologoustoendogenousgenesoftenresultedinplantswithbothgenessuppre