资源描述：
《南大2419 x 望水白群体中PPO活性的QTL定位》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、南大2419x望水白群体中籽粒多酚氧化酶活性的QTL定位吴纪中1蔡士宾1马正强2（1．江苏省农科院粮食作物研究所，南京，210014；2.南京农业大学应用植物基因组实验室，南京，210095）摘要：多酚氧化酶(polyphenoloxidase，PPO)是引起小麦面粉及面制品颜色褐变的主要原因。本文利用基于L-DOPA方法对南大2419×望水白F9:10代重组自交系（recombinationinbredline，RIL）群体在3个种植环境的籽粒进行了PPO活性分析，并结合该群体已构建的覆盖小麦基因组3360cM，包括
2、408个标记33个连锁群的遗传框架图，对控制小麦籽粒PPO活性的QTLs分别进行了单向方差分析、区间作图和复合区间作图分析。结果表明，14个染色体区域与该群体籽粒PPO活性极显著相关，分别位于第一、二同源群以及3B、4B、5B、5D、6B和7B上，其中位于染色体2AL的Xgwm312和2DL的Xsts49.2在3个环境中均与PPO活性极显著相关；区间作图和复合区间作图发现控制该群体籽粒PPO活性的主效QTL有2个，分别位于染色体2AL的Xgwm312-Xgwm47.32区间和2DL的Xsts49.2-Xcfd16区间，
3、贡献率分别为10.9%～19.1%和13.6%～32.9%，这两个主效QTL均来自高PPO活性亲本南大2419。关键词：普通小麦；多酚氧化酶(PPO)；分子标记；QTL分析MappingQTLsassociatedwithPolyphenolOxidaseActivityintheNanda2419xWangshuibaiRILpopulationWUJi-zhong1,CAIShi-bin1,MAZheng-qiang2(1.InstituteofFoodCrop,JiangsuAcademyofAgricultur
4、alScience,Nanjing210014,China;2.TheAppliedPlantGenomicsLab,CropgenomicsandBioinformaticsCenter,NanjingAgriculturalUniversity,Jiangsu210095,China)Abstract：Theenzymeactivityofpolyphenoloxidase(PPO)inkernelhasbeenrelatedtoundesirablebrowndiscolorationofbreadwheat(T
5、riticumaestivumL.)basedend-products,particularlyforAsiannoodles.DevelopingwheatcultivarswithloworzeroPPOactivityisthebestapproachtoreducetheundesirabledarkening.ToidentifygenesforPPOactivity,therecombinationinbredline(RIL)populationderivedfromNanda2419xWangshuib
6、aiandtheparentswereassessedbasedonL-DOPAassaysinthreetrials.Alinkagemapcoveringover3360cMofwheatgenomewasusedforQTLanalysis,andfourteenchromosomeregionswereidentifiedsignificantlyassociatedwithPPOactivitybyone-wayANOVA,whichlocatedonwheatchromosomesgroup1,2,3B,4
7、B,5B,5D,6Band7B.AmongoftheseQTLmarkers,Xgwm312andXsts49.2werehighlysignificantlyassociatedwithPPOactivityinthreetrials,whichlocatedonchromosomes2ALand2DL.Withmethodsofsimpleandcompositeintervalmapping(CIM),twoputativemajorQTLsweredetectedonchromosome2ALand2DL,ac
8、countingfor10.9%～19.1%and13.6%～32.9%ofthephenotypicvarianceacrossthreeenvironments,respectively.Keywords：Commonwheat;TriticumaestivumL.；Polyphenoloxidase;Molecularmar
