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1、作物学报ACTAAGRONOMICASINICA2009,35(6):1038−1043http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2009.01038利用永久F2群体定位小麦株高的QTL*王岩李卓坤田纪春山东农业大学/国家作物生物学重点实验室小麦品质育种室,山东泰安271018摘要:为研究小麦株高的遗传机制,利用DH群体构建了一套包含168个杂交
2、组合的小麦永久F2群体,并于2007年种植于山东泰安和山东聊城。构建了一套覆盖小麦21条染色体的遗传连锁图谱,并利用该图谱的324个SSR标记对小麦株高进行QTL定位研究,使用基于混合线性模型的QTLNetwork2.0软件进行QTL分析。在永久F2群体中定位了7个株高QTL,包括4个加性QTL,1个显性QTL,1对上位性QTL,共解释株高变异的20%,其中位于4D染色体的qPh4D,具有最大的遗传效应,贡献率为7.5%;位于2D染色体显性效应位点qPh2D,可解释1.6%的表型变异;位于5B~6D染色体上位效应位点,
3、可解释1.7%的表型变异。还发现加性效应、显性效应和上位效应对小麦株高的遗传起重要作用,并且基因与环境具有互作效应。关键词:小麦;永久F2群体;株高;数量性状位点DetectionofQuantitativeTraitLociforPlantHeightUsinganImmortalizedF2PopulationinWheat*WANGYan,LIZhuo-Kun,andTIANJi-ChunGroupofQualityWheatBreedingofKeyLaboratoryofCropBiology/Shandon
4、gAgriculturalUniversity,Tai’an271018,ChinaAbstract:Tostudythegeneticmechanismofwheatplantheight,asetofdoubledhaploid(DH)lineswereusedtoconstructanimmortalizedF2(IF2)populationcomprising168differentcrosses.TheIF2populationwasevaluatedforplantheightin2007’cropping
5、seasonsinTaianandLiaocheng,Shandongprovince.Linkagemapwasconstructedwith324SSRmarkerscoveringthewholewheatgenome,including284SSR,37ESTsloci,1ISSRlociand2HMW-GSloci,wasconstructed.Thislinkagemapcoveredatotallengthof2485.7cMwithanaveragedistanceof7.67cMbetweenadja
6、centmarkers.QTLanalyseswereperformedusingthesoftwareQTLNetworkversion2.0basedonthemixedlinearmodelatP<0.05.FouradditiveQTLs,1dominanceQTLandpairofepistaticQTLsweredetected,thetotalQTLeffectsdetectedfortheplantheightexplained20%ofthephenotypicvariation.OneQTLqPh4
7、Dforplantheightwasidentifiedonchromosome4D,wasidentifiedonchromosome2D,explaining7.5%ofthephenotypicvariances.DominanceeffectlociqPh2Dwasidentifiedonchromosome2D,explaining1.6%ofthephenotypicvariances;epistaticeffectsoflociwasidentifiedonchromosome5B–6D,explaini
8、ng1.7%ofthephenotypicvariances.Theresultsindicateadditiveeffects,dominanceeffectsandepistaticeffectsareimportantingeneticsofwheatforplantheight,whicharealsosubjectedt
