Efficient production of Agrobacterium rhizogenes-transformed版本

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1、PlantCellTissOrganCult(2012)109:491–500DOI10.1007/s11240-012-0113-1ORIGINALPAPEREfficientproductionofAgrobacteriumrhizogenes-transformedrootsandcompositeplantsinpeanut(ArachishypogaeaL.)LiliGeng•LihongNiu•PeterM.Gresshoff•ChanglongShu•FupingSong•DafangHuang•JieZhangReceived:7July2011/Accepted:30Decem

2、ber2011/Publishedonline:28January2012ÓSpringerScience+BusinessMediaB.V.2012AbstractRecalcitranceofmostlarge-seededlegumes,suchKeywordsAgrobacteriumrhizogenes
Peanut
aspeanut,toregenerationandgenetictransformationhasTransformation
Microinjection
cry8Ea1genehamperedstudiesongenefunctionandeffortsforge

3、neticimprovement.Agrobacteriumrhizogenes-mediatedtransfor-AbbreviationsmationprovidesasystemforrapidandefficienttransformationDIRCDaysofinducedrootsemergingafterofplanttissues.Inthisstudy,embryonicaxesalongwithco-cultivationcotyledonsofpeanutwereinjectedwithasuspensioncultureofNCNon-transgeniccontrol

4、A.rhizogenesusingmicrolitersyringes.Theinfluenceofsev-eralfactorssuchasplantgenotype,A.rhizogenesculturestage,co-cultureperiodofA.rhizogenes,andacetosyringonecon-Introductioncentrationintheco-cultivationmediumhavebeenevaluated.ItisfoundthatA.rhizogenes-mediatedtransformationofpeanutWorldpeanutproduct

5、ionreached34.42millionmetrictonsinisgenotype-independent.Upto61%transformationwas2010(USDA2010),beingChina,IndiaandUnitedstatestherecordedwhenembryonicaxeswereco-cultivatedwithleadingproducingcountries.Peanuts,whicharegoodsources59107A.rhizogenescellsfromlogarithmicphasefor2daysofproteins,calories,v

6、itamins,andminerals,haveshownhighonco-culturemediumcontaining50lmoll-1acetosyringone.potentialtoreducehungerandmalnutritioninThirdWorldCompositeplantswithtransgenicrootswereharvestedaftercountries(Enserink2008).However,yieldsofpeanutsare45daysoftreatment.Furthermore,thismethodwasappliedtoseriouslyaf

7、fectedbysubterraneaninsects,fungalandbacte-assesstheinsecticidalactivityofasyntheticcry8Ea1generialdiseases(VargasGiletal.2008).RecalcitranceofmostagainstHolotrichiaparallelaintransgenicrootsofpeanut.