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ID:40230161
大小:20.12 MB
页数:66页
时间:2019-07-27
《生物化学与分子生物学PPT图片素材-Science_Slide》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、生物化学与分子生物学PPT素材可根据需要选择取舍Metabolismof5-fluorouracil(5-FU)5-FUCarac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridinephosphorylase5-FluorodeoxyuridineThymidinephosphorylaseOrotatephosphoribosyltransferaseThymidinekinase5-FUTP5-FdUTPRNADNA5’-nucleotidase5’-nucleotidaseUridinekinaseATPADPATPADPPRPPWes
2、ternBlotting1.ResolveproteinsamplesonSDS-PAGE6.IncubatetheblotwithchemiluminescentHRPsubstrateandexposetofilm2.ElectrophoreticallytransferfractionatedproteinsfromgelontoPVDFmembrane3.Blockthemembranewithneutralprotein(BSAormilkcasein)4.Incubatethemembranewithprimaryantibodyspecifictotar
3、getprotein5.IncubatethemembranewithHRP-labeledsecondaryantibodyspecifictoprimaryantibodyCholesterolSterolToolkitPregnenolonProgesteronAldosteronCortisolCorticosteroneAndrostendioneTestosteronEstriol17-Estradiol1,25-DihydroxycholecalciferolCholicacidCovalentmodificationofproteinsPoly(ADPribos
4、yl)Polymerase(PARP)Poly(ADPribosyl)polymerase(PARP)NADNicotinamidePoly(ADPribosyl)polymerase(PARP)Poly(ADPribosyl)chainelongationandbranchingNicotinamideNADNADDNApolymeraseDNApolymeraseDNApolymeraseOligosacharyltransferaseN-glycosylationpathwayinERMann-P-DolicholtransferasesFlippaseDol
5、icholPDolicholPPDolicholPPDolicholPPDolicholPPGlcNAc-PtransferaseGlcNActransferaseDolicholPDolicholPPDolicholPPGDP-MannosyltransferasesGlc-P-DolicholtransferasesDolicholPDolichol-pyrophofphataseCytosolER40S60SUTPCTPTMPPyrimidineNucleotideInterconversionsUMPATPADPH2OUDPNucleosidemonophosphat
6、ekinaseATPADPNucleosidediphosphatekinaseATPADPCTPsynthaseRibonucleotidereductasedeoxyUDPNucleosidediphosphatephosphohydrolasedeoxyUMPThymidylatesynthaseN5,N10-MethyleneTHFDHFTTPNucleosidemonophosphatekinaseNucleosidediphosphatekinaseMyo-Inositol-3-phosphateIns(3)P1Biosynthesisandmet
7、abolismofphosphoinositidesGlucose6-pgosphateIns(3)P1synthaseIns(3)P1phosphataseMyo-InositolInsPtdInssynthasePtdInsMyo-inositolkinasePtdIns-4-PPtdIns4-kinasePtdIns5-kinasePtdIns-4,5-P2PI3KPtdIns-3,4,5-P3PLCPLCPI3KPtdIns-
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