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ID：59716745
大小：12.28 MB
页数：66页
时间：2020-11-20
《生物化学与分子生物学PPT图片素材说课材料.ppt》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、生物化学与分子生物学PPT图片素材WesternBlotting1.Resolveproteinsamples onSDS-PAGE6.Incubatetheblotwith chemiluminescentHRP substrateandexposetofilm2.Electrophoreticallytransfer fractionatedproteinsfrom gelontoPVDFmembrane3.Blockthemembrane withneutralprotein (BSAormilkcasein)4.Incubatethe
2、membrane withprimaryantibody specifictotargetprotein5.Incubatethemembrane withHRP-labeledsecondary antibodyspecifictoprimary antibodyCholesterolSterolToolkitPregnenolonProgesteronAldosteronCortisolCorticosteroneAndrostendioneTestosteronEstriol17-Estradiol1,25-Dihydroxychol
3、ecalciferolCholicacidCovalentmodificationofproteinsPoly(ADPribosyl)Polymerase(PARP)Poly(ADPribosyl) polymerase (PARP)NADNicotinamidePoly(ADPribosyl) polymerase (PARP)Poly(ADPribosyl) chainelongation andbranchingNicotinamideNADNADDNApolymeraseDNApolymeraseDNApolymeraseOli
4、gosacharyl transferaseN-glycosylationpathwayinERMann-P-DolicholtransferasesFlippaseDolicholPDolicholPPDolicholPPDolicholPPDolicholPPGlcNAc-P transferaseGlcNAc transferaseDolicholPDolicholPPDolicholPPGDP-Mannosyl transferasesGlc-P-Dolichol transferasesDolicholPDolichol-pyrop
5、hofphataseCytosolER40S60SUTPCTPTMPPyrimidineNucleotideInterconversionsUMPATPADPH2OUDPNucleoside monophosphate kinaseATPADPNucleoside diphosphate kinaseATPADPCTP synthaseRibonucleotide reductasedeoxyUDPNucleoside diphosphate phosphohydrolasedeoxyUMPThymidylate synthaseN5,N10
6、-MethyleneTHFDHFTTPNucleoside monophosphate kinaseNucleoside diphosphate kinaseMyo-Inositol-3-phosphate Ins(3)P1BiosynthesisandmetabolismofphosphoinositidesGlucose6-pgosphateIns(3)P1synthaseIns(3)P1phosphataseMyo-Inositol InsPtdIns synthasePtdInsMyo-inositol kinasePtdIns-4-
7、PPtdIns 4-kinasePtdIns 5-kinasePtdIns-4,5-P2PI3KPtdIns-3,4,5-P3PLCPLCPI3KPtdIns-3,4-P2Ins-1,3,4-P3Ins-1,3,4,5-P4Ins-1,4,5-P3PLCPTENPTENIns-1,4-P3PTENPTENFattyacid synthase-ketoacyl ACP-synthaseFattyacidssynthesisAcetylCoA carboxylaseATPADP+PiAcetylCoAMalonylCoACitrateOxalo
8、acetateATPADP+PiCitratelyaseAcetyl transacylaseMalonyl transacylasePantetheine-ke
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