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ID:37375874
大小:4.82 MB
页数:12页
时间:2019-05-12
《生物化学与分子生物学PPT图片素材-Science-Sl》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、AntibodyToolkit(IgG)αhelixVimentinActinandmyosinCoiled-coilMicrotubule6nm10nm25nmNucleosomes2ACACDDR1DDR1DDR176123454356217CD45PTPMetabolismof5-fluorouracil(5-FU)5-FUCarac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridinephosphorylase5-FluorodeoxyuridineThymidinephosphorylaseOr
2、otatephosphoribosyltransferaseThymidinekinase5-FUTP5-FdUTPRNADNA5’-nucleotidase5’-nucleotidaseUridinekinaseATPADPATPADPPRPPWesternBlotting1.ResolveproteinsamplesonSDS-PAGE6.IncubatetheblotwithchemiluminescentHRPsubstrateandexposetofilm2.Electrophoreticallytransferfra
3、ctionatedproteinsfromgelontoPVDFmembrane3.Blockthemembranewithneutralprotein(BSAormilkcasein)4.Incubatethemembranewithprimaryantibodyspecifictotargetprotein5.IncubatethemembranewithHRP-labeledsecondaryantibodyspecifictoprimaryantibody
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