Regeneration of critical bone defects with anionic collagen matrix as scaffolds

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1、JMaterSci:MaterMedDOI10.1007/s10856-013-4980-8RegenerationofcriticalbonedefectswithanioniccollagenmatrixasscaffoldsFu´lvioBorgesMiguel•AryondeAlmeidaBarbosaJu´nior•FabianaLopesdePaula•IsabelaCerqueiraBarreto•GilbertoGoissis•FabianaPaimRosaReceived:11December2012/Accepted:10

2、June2013ÓSpringerScience+BusinessMediaNewYork2013AbstractTheaimofthisstudywastomakeahisto-ofcriticalbonedefectwasmoreevidentinanioniccolla-morphometricevaluationoftheosteogenicpotentialofgenwithoutcrosslinking(ACSH).anioniccollagenmatrixasscaffolds;eithercrosslinkedinglutar

3、aldehydeornotcross-linkedand,implantedincriti-calbonedefectsinratcalvaria.Seventy-tworatswere1Introductionrandomlydistributedinthreegroups:anioniccollagenscaffoldstreatedfor24hofselectivehydrolysis(ACSH);TheresearchersofTissueBioengineeringAreahaveusedanioniccollagenscaffol

4、dstreatedfor24hofselectivebiomaterials,cells,andgrowthfactorsforcreatingartificialhydrolysisand5minofcrosslinkinginglutaraldehydeorgansandtissues,orbiologicalsubstitutesforimproving0.05%(ACSHGA);emptybonedefect(Control),evalu-orrestoringlosttissuefunction[1].Biomaterialsofth

5、eatedatthebiologicalpointsof15,45,90and120days.scaffoldtypeusedintheformofathreedimensional(3D)Theresultsshowedthatthebiomaterialsimplantedwerematrix,provideasubstrateforthecellsmigration,prolif-biocompatibleandshowedahighosteogenicpotential.eration,differentiation,anddepos

6、itanewextracellularThesebiomaterialspresentedaspeedofbiodegradationmatrixandformnewbloodvesselsessentialfortissuecompatiblewithboneneoformation,whichwasshowntoregeneration.AccordingtoO’Brienetal.[2]naturalscaf-beassociatedwithangiogenesisinsidethescaffoldsatallfoldsplayanim

7、portantroleintissueregenerationbecausebiologicalpoints.Thepercentageofmineralizationoftheypotentiallymimicthevariousfunctionsofextracel-ACSH(87%)differedstatisticallyfromthatfoundinlularmatrix(ECM).ACSHGA(66%).ItwasconcludedthattheregenerationAmongthenaturalpolymersusedfort

8、hemanufactureofthese3Dmatrices,theTypeIcollageniswidelyusedasarawmaterialduetoitsl