Effect of solid freeform fabrication

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1、JMaterSci:MaterMed(2013)24:1053–1065DOI10.1007/s10856-013-4867-8Effectofsolidfreeformfabrication-basedpolycaprolactone/poly(lactic-co-glycolicacid)/collagenscaffoldsoncellularactivitiesofhumanadipose-derivedstemcellsandratprimaryhepatocytesJin-HyungShim•

2、ArthurJoonKim•JuYoungPark•NamwooYi•InhyeKang•JaesungPark•Jong-WonRhie•Dong-WooChoReceived:9August2012/Accepted:18January2013/Publishedonline:22February2013ÓSpringerScience+BusinessMediaNewYork2013AbstractHighlybiocompatiblepolycaprolactone(PCL)/sponge-ty

3、pecollagenscaffoldwhoseweakmechanicalprop-poly(lactic-co-glycolicacid)(PLGA)/collagenscaffoldsinertieswereregardedasacriticaldrawback.ProliferationandwhichthePCL/PLGAcollagensolutionwasselectivelydis-osteogenicdifferentiationofhASCswerepromotedsignifi-pen

4、sedintoeveryotherspacebetweenthestrutswerefabri-cantlyingroup3comparedtogroups1and2.Inaddition,wecatedusingsolidfreeformfabrication(SFF)technology,aswefoundthattheviabilityandalbuminsecretionabilityofratdescribedpreviously.Theobjectiveofthisstudywastopri

5、maryhepatocyteswerehighlyretainedfor10daysingroupevaluateandcomparethePCL/PLGA/collagenscaffolds3butnotgroup1.Interestingly,hepatocyteaggregation,(group3)withPCL/PLGA-onlyscaffolds(group1)andPCL/whichenhanceshepaticfunctionthroughcell–cellinterac-PLGAsca

6、ffoldswithcollagenbythedip-coatingmethodtions,wasobservedparticularlyingroup3.Inconclusion,(group2)usinghumanadipose-derivedstemcells(hASCs)group3,inwhichthecollagenwasselectivelydispensedintheandratprimaryhepatocytes.Theselectivelydispensedcol-3Dspaceof

7、theporousPCL/PLGAframework,willbealagenformedathree-dimensional(3D)networkofnanofiberspromising3Dscaffoldforculturingvariouscelltypes.ingroup3,asobservedbyscanningelectronmicroscopy.Thecompressivestrengthandmodulusofgroup3wereapproxi-mately140and510timesh

8、igher,respectively,thanthoseofa1IntroductionThree-dimensional(3D)scaffoldsarewidelyrecognizedasElectronicsupplementarymaterialTheonlineversionofthisessentialforreplacementandrepairofdamagedtissuesorarticle(doi:10.1007/s108