Analysis of elevated temperature-induced inhibition of photosystem II using chlorophyll a fluorescence induction kinetics in wheat leaves (Triticum aestivum)

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1、PlantBiologyISSN1435-8603RESEARCHPAPERAnalysisofelevatedtemperature-inducedinhibitionofphotosystemIIusingchlorophyllafluorescenceinductionkineticsinwheatleaves(Triticumaestivum)S.Mathur,A.Jajoo,P.Mehta&S.BhartiSchoolofLifeScience,DeviAhilyaUniversity,Indore,MP,IndiaKeywordsABSTRACTChlafluorescenc

2、e;elevatedtemperature;photosystemII;wheat.Wheatisthemajorcropplantinmanypartsoftheworld.Elevatedtemperature-inducedchangesinphotosyntheticefficiencywerestudiedinwheat(T.aestivum)CorrespondenceleavesbymeasuringChlafluorescenceinductionkinetics.DetachedleaveswereA.Jajoo,SchoolofLifeScience,DeviAhil

3、yasubjectedtoelevatedtemperaturestressof35°C,40°Cor45°C.ParameterssuchUniversity,Indore452017,MP,India.asFv/Fm,performanceindex(PI),andreactioncentretoabsorbanceratioE-mail:anjanajajoo@hotmail.com(RC/ABS)werededucedusingradialplotsfromfluorescenceinductioncurvesobtainedwithaplantefficiencyanalyse

4、r(PEA).ToderivepreciseinformationonEditorfluorescenceinductionkinetics,energypipelineleafmodelswereplottedusingbio-R.Leegoodlyzerhp3software.At35°C,therewasnoeffectonphotosyntheticefficiency,includingtheoxygen-evolvingcomplex,andthedonorsideofPSIIremainedactive.Received:5November2009;Accepted:2At

5、40°C,activitywasreducedby14%,whileat45°C,aKintermediatestepwasDecember2009observed,indicatingirreversibledamagetotheoxygen-evolvingcomplex.Thisanal-ysiscanbeusedtorapidlyscreenforvitalityandstresstolerancecharacteristicsofdoi:10.1111/j.1438-8677.2009.00319.xwheatgrowinginthefieldunderhightempera

6、turestress.firsttoreportthat,followingilluminationofadark-adaptedINTRODUCTIONphotosyntheticsample,ChlafluorescenceemissionisnotWheatiscommerciallyoneofthemostimportantcrops,constantbutexhibitsafastrisetoamaximumfollowedbyaespeciallyintropicalcountries.However,tropicalcountriesdeclinetoasteadystat

7、eoversomeminutes.Chlorophyllflu-alsohavehightemperaturesduringthegrowingseason.Croporescencehasbeenusedtoprovidearapid,non-destructiveyieldisgreatlyaffectedbyelevatedtemperaturestressandhasdiagnosticmethodfordetectingandquantifying