绿原酸纳米脂质体制备与抑菌性分析

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1、622014,Vol.35,No.20食品科学※工艺技术绿原酸纳米脂质体制备与抑菌性分析徐贤柱，魏允，饶华，王曼莹*（1.江西亚热带植物资源保护与利用重点实验室，江西南昌330022；2.江西师范大学生命科学学院，江西南昌330022）摘 要：目的：研究绿原酸纳米脂质体制备及其抑菌性。方法：采用薄膜超声法制备绿原酸纳米脂质体，并用扫描电镜和粒度仪分析测定其形貌及粒径，考察膜材比、药脂比及超声时间对包封率的影响，最后对其体外稳定性和抑菌能力进行评价。结果：胆固醇与卵磷脂质量比1∶8、绿原酸与膜材质量比1∶10、超声时间15min所制备的绿原酸纳米脂质体呈椭

2、圆形，形态规整，粒径在110nm左右，分散性良好，包封率和载药量最高分别达到87.5%和36%；紫外测试表明绿原酸被成功包覆在脂质体中；体外缓释实验表明，在24h和14d中绿原酸纳米脂质体均释放稳定；在抑菌实验中，绿原酸纳米脂质体与绿原酸和四环素相比具有更持久的抑菌能力。结论：采用薄膜超声法制备的绿原酸纳米脂质体具有很好的形貌和分散性能，也具有很好持续抑菌能力。关键词：绿原酸；脂质体；制备；抑菌PreparationandAntibacterialEffectofChlorogenicAcidNanoliposomeXUXian-zhu,WEIYun,R

3、AOHua,WANGMan-ying*(1.JiangxiProvincialKeyLaboratoryofProtectionandUtilizationofSubtropicalPlantResources,Nanchang330022,China;2.CollegeofLifeSciences,JiangxiNormalUniversity,Nanchang330022,China)Abstract:Objective:Toinvestigatethepreparationandantibacterialeffectofchlorogenicaci

4、d(CA)nanoliposome.Methods:CAnanoliposomeswerepreparedbyfilm-ultrasonicdispersionmethodandtheproductswerecharacterizedwithscanningelectronmicroscopy(SEM)andparticlesizeanalyzer.Theeffectsofcholesterol/lecithinmassratio,CA/film(lecithin+cholesterol)massratioandultrasonicationtimeont

5、heencapsulationefficiencyofliposomeswereinvestigated.Finally,thestabilityandthebacteriostasisabilityofthepreparedliposomeswereevaluated.Results:Oval-shapedliposomeswerepreparedbyultrasonicationfor15minatacholesterol/lecithinmassratioof1:8andaCA/filmratioof1:10,whichshowedregularmor

6、phology,aparticlesizeofapproximately110nmindiameter,gooddispersity,anencapsulationefficiencyofupto87.5%,andadrugloadingofupto36%.UVspectroscopyshowedthatCAwasencapsulatedsuccessfullyintonanoliposomes.Invitrotestshowedthatthedrugcouldbesustained-releasedfromliposomeswithoutaburstre

7、leaseandwithstabilityfor24hand14days.ComparingwithCAandtetracycline,thenanoliposomeshadbetterbacteriostasisability.Conclusion:CAnanoliposomespreparedbyfilm-ultrasonicdispersionmethodhavearegularshape,gooddispersionabilityandpersistentantibacterialability.Keywords:chlorogenicacid;n

8、anoliposomes;preparation;antibacterialac