Biosensors and Bioelectronics生物传感器化学

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1、BiosensorsandBioelectronics86(2016)454–458ContentslistsavailableatScienceDirectBiosensorsandBioelectronicsjournalhomepage:www.elsevier.com/locate/biosUltrasensitiveelectrochemicaldetectionofmiRNA-21byusinganiridium(III)complexascatalysta,bbcbbXiangminMiao,WanheWang,Tian

2、shuKang,JinbiaoLiu,Kwok-KeungShiu,cb,nChung-HangLeung,Dik-LungMaaSchoolofLifeScience,JiangsuNormalUniversity,Xuzhou221116,PRChinabDepartmentofChemistry,HongKongBaptistUniversity,KowloonTong,HongKong,ChinacStateKeyLaboratoryofQualityResearchinChineseMedicine,InstituteofC

3、hineseMedicalSciences,UniversityofMacau,Macao,ChinaarticleinfoabstractArticlehistory:TheultrasensitiveelectrochemicaldetectionofmiRNA-21wasrealizedbyusinganovelredoxandcat-Received5May2016alytic“all-in-one”mechanismwithaniridium(III)complexasacatalyst.Toconstructsuchase

4、nsor,aReceivedinrevisedformcaptureprobe(CP)wasfirstlyimmobilizedontothegoldelectrodesurface.InthepresenceofmiRNA-21,27June2016asandwichedDNAcomplexcouldformbetweenCPandamethyleneblue(MB)labeledG-richdetectionAccepted1July2016þprobemodifiedontoagoldnanoparticle(AuNP)surfac

5、e(DP-AuNPs).UponadditionofK,thestructureAvailableonline2July2016ofDPchangedtoaG-quadruplex.Then,theiridium(III)complexcouldselectivelyinteractwiththeKeywords:G-quadruplex,catalyzingthereductionofH2O2,whichwasaccompaniedbyanelectrochemicalsignalIridium(III)complexchangeu

6、singMBasanelectronmediator.Underoptimalconditions,theelectrochemicalsignalofMBmiRNA-21reductionpeakwasproportionaltomiRNAconcentrationintherangefrom5.0fMto1.0pM,withaElectrochemicalbiosensordetectionlimitof1.6fM.Inaddition,satisfactoryresultswereobtainedformiRNA-21detec

7、tioninhumanserumsamples,indicatingapotentialapplicationofthesensorforbioanalysis.&2016ElsevierB.V.Allrightsreserved.1.Introductiontheirsimplicity,easyminiaturization,lowcostandhighsensitivity(Chengetal.,2009;Dengetal.,2014;Liuetal.,2013;Rafiee-PourmiRNAsareagroupofshort(

8、approximately22nucleotides)etal.,2016;Wenetal.,2012).andendogenouslyexpressednoncodingRNAs,whichplaycrucialRec