Comparison of In Vitro and In Vivo Screening Models for Androgenic英文文献资料

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1、TOXICOLOGICALSCIENCES89(1),173187(2006)doi:10.1093/toxsci/kfj009AdvanceAccesspublicationOctober12,2005ComparisonofInVitroandInVivoScreeningModelsforAndrogenicandEstrogenicActivities,1,EdwinSonneveld,*JacobaA.C.Riteco,*HendrinaJ.Jansen,*BartPieterse,*AbrahamBrouwer,*Willem

2、G.Schoonen,andBartvanderBurg**BioDetectionSystemsB.V.,Amsterdam,TheNetherlands;InstituteforEnvironmentalStudies,VrijeUniversiteitAmsterdam,Amsterdam,TheNetherlands;andDepartmentofPharmacology,NVOrganon,Oss,TheNetherlandsReceivedAugust25,2005;acceptedSeptember27,2005regula

3、torypathwayscaneasilybemodifiedbypharmacolog-DownloadedfromIdentificationofnuclearreceptor–mediatedendocrineactivitiesical,environmental,and/ordietaryagents.Becauseofthis,isimportantinavarietyoffields,rangingfrompharmacologicalsteroidsorsteroid-mimickingcompoundsareappliedin

4、manyandclinicalscreening,tofoodandfeedsafety,toxicologicalfields,makingidentificationoftheendocrineactivityofthesemonitoring,andriskassessment.Traditionallyanimalstudiescompoundsimportant.Analytical-chemicalandimmunologi-suchastheHershbergerandAllen-Doisytestsareusedforthea

5、ssessmentofandrogenicandestrogenicpotencies,respectively.calmethodsarecommonlyusedtodetectsteroidsinfoodandhttp://toxsci.oxfordjournals.org/Toallowfastanalysisoftheactivitiesofnewchemicals,foodfeed,clinicalpractice,environmentalsamples,ordopingadditives,andpharmaceuticalc

6、ompounds,high-throughputcontrol.Thesemethodshavethedrawbackthattheyonlyscreeningstrategieshavebeendeveloped.Here,apanelofmainlyquantifythecompoundofinterestandarenotabletodeterminesteroidalcompounds,screenedindifferentinvitroassays,wasbiologicalactivityofunknowncompoundso

7、rtheirmetabolites,ÒcomparedwithtwohumanU2-OScellline–basedCALUXthisincontrasttobiologicalassays.(ChemicallyActivatedLUciferaseeXpression)reportergeneBioassaysinrats,mice,orrabbitsweredevelopedalongassaysforandrogens(ARCALUX)andestrogens(ERaCALUX).timeagotodeterminetheendo

8、crineactivityofcompounds.CorrelationsfoundbetweenthedataofthesetwoCALUXImportantexamplesaretheas