IGF-IR在人的不同肝细胞上表达和定位与阻断IGF-IR对HepG2细胞生长影响的实验分析

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1、兰州大学硕士学位论文ExpressionandLocalizationofIGF-IReceptorinHepG2CellsandChangLiverCellsandEffectofAnti-IGF·IReceptorMonoclonalAntibodyonGrowthofHepG2CellsABSTRACTPartone：ExpressionandlocalizationofIGF-IreceptorinHepG2cellsandChanglivercellsobjectiveTostudytheexpressionandlocalizationofI

2、GF—IreceptorOOF．IR)inHepG2cellsandChanglivercellsMethodsThecellculturemethodwasusedtopreparethecoverslipsofHepG2cellsandChangliverceils，andthentheexpressionandlocalizationofIGF—IRinHepG2cellandChanglivercellWasdetectedbyimmunohistochemistryResults(1)BothofChangliverceilsandHepG2c

3、ellsexpressIGF-Ireceptor(IGF—JR)，andIGF—IRstainingWaSobservedinbothcellularmembraneandcytoplasmofChanglivercellsandHep02cells；(2)TheIGF—IRstainingofChanglivercellspresenteddilutebrown，andthmofHepG2cellspresenteddeepbrown．Comp积withChanglivercells，HepG2cellsexpressedIGF—Ireceptormo

4、restrong．Conclusions[GF—mwassurelyexpressedinnormalhumanhepatocytesChangliver，whichimpliesthatIGF-IRmayplayapivotalroleonthebiologicalbehaviorsofthenormalhepatocytes．ComparedwiththatofChanglivercell，theexpressionlevelofIGF—IR，Ⅲ兰州大学硕士学位论文whichw-aslocatedinthecellularmembranesofbot

5、hcells，washigherinthecellmembr柚esofHepG2cells，whmhsuggeststhattheoverexpressionofIGF—IRmayberelatedtothetransformationofhepatocytesfromthenormaltothemalignant．Inanotherwords．overexpressionoflGF—IRisrelatedtothebiologicalbehaviorsofhumanmaligimnciesKeywordsnormalhepmoc”e；hepatocel

6、lularcarcinomacells；insulin-likegrowthfactor-Ireceptor；expression；localizationParttwo：Effectofanti-IGF—IreceptormonoelonalantibodyongrowthofHepG2ceHsobjectiveToobservetheeffectofanti—IGF—Ireceptormonoclonalantibody(aiR3)onthegrowthandapoptosisofHepG2cells．MethodsTheinfluenceofaiR

7、3ontheproliferationandapoptosisofHepG2cellswereexaminedbythe3-(4，5-dimethytthiazol-2·y1)-2，5一diphenyltetrazoliumbromide(MTT)assayandelectronmicroscopy，respectively．Theflowcytometry(FCM)wasappliedfortheanalysisofthecellcycleandapoptosisofHepG2cellsculturedinmediawithandwithout10％N

8、BCS．Morphologicalchangesofapoptosiswereo