ngal在单侧输尿管结扎大鼠肾脏的表达与促肝细胞生长素对其影响

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1、英文摘要obstmction(UUO)ratkidney，andt11ecorrelationbetweenHGF，NGALandE．cadherein(E．cad)under也enerventionofexogenousHGF’and也enexploret11emechaJlismbetweenHGF’NGALandtheprocessof‘cdamage．repan6brosis’’，t11usprovidenewideasforthetreatmentofrenalfibrosis．Method：72cleanlevelhealt}lymaleSDrats，bodywei出

2、200士20g，wererandomlydividedintothreegroups：(1)shamoperation铲oup(sh锄group)：onlysepaI．atetheleRureter，noligatedorcut，In仃aperitonealinjection(IP)ofsalinedaily；(2)吼ilateralureteral0bstmction一0perationgroup(UUOgroup)：meleRureterwasligatedandcut，I腑aperitonealinjectionofsalinedaily；(3)h印atoc”egrowth

3、promotingf-acto—nterventiongroupQHGFgroup)：me1eRureterwasligatedandcut，pHGFO．5m删daybyI．Pdaily．RatsineaChgroupreceiVedintr印eritoneal埘ection舶mldaybeforemeoperationurltilthe14mpostoperative￡lay，inwhichtheVolumeofsalineinSh锄groupaIldUUOgroupwaSequaltothepHGF伊oup．Duringtheexperimentratswereprovide

4、dwith仔eefoodandwater．6ratswererandomlyselectedoutofeachgroupInthe1st，4th，7th，14thdaysaRertheoperation．TheleRl(idneyswereharVestedandmadeintosections．RespeCtively，tllepatllologicalchangesofrenaltissuewithhematoxylineosin(HE)a11dMassonstainingwereobsen，edunderthe1ightmicroscope；NGAL，E．cadandHGF

5、expressionwereexaminedbyImmunohistochemicalmethod．7IheresultswereanalyzedusingmeimageanalysissystemIPP(Image—ProPlus)forsemi．quamitatiVeanalysis．Allthedatawereexpressedasmean士standarddeviation，singlef．actoranalysisofVariancewasused锄ongmethreegroups，twoiIldependents锄plest-testbetweentwo铲oups，a

6、ndnon．parametrictestsforthedatawhichdidnotmeetthenomalit)，andhomogenei钞ofv撕ance．S洲Stic出anaJysisso脚areSPSSl7．Ow2Lsusedfordatastatistics，witllP

7、ubulointerstitialareaaIldthecontentofrenalinterstitialc011agenfiber5英文摘要increased鲈aduallywithureteralobstmctionprolonged，whichsuggestedanincreasingleVelofrenalinterStitialfibrosis．ComparedwimUUO鲈oup，thehistopathologicalchangeswerea11eVi2吨edin