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1、Anal.Chem.2010,82,441446SynthesisofCore-ShellSurface-EnhancedRamanTagsforBioimagingXiangjiangLiu,MariaKnauer,NataliaP.Ivleva,ReinhardNiessner,andChristophHaisch*ChairforAnalyticalChemistry,TechnischeUniversita¨tMu¨nchen,Marchioninistrasse17,D81377Munich,GermanyWepresentarapidandstraig
2、htforwardprocedurefortheThesecond,improvedapproachistocoverthesurfaceofsynthesisofcore-shellsurface-enhancedRamanscatter-nanoparticleswithalayerofsilicaorpolymers,inwhichtheing(SERS)tags.SERStagsarecombinationsofRamanRamanreportermoleculesareembedded(so-calledcore-shellactivedyeswithm
3、etallicnanoparticlesinducingthe5SERStags).ThefirstattemptwasmadebyDoeringetal.andenhancement.Becauseoftheirsignificantlyhigherstabil-6Mulvaneyetal.ThesilicashellsprotecttheSERStagsfromitytoenvironmentalconditions,themajorityofSERStagsaggregationandfacilitatefunctionalization.Evensomemor
4、earedesignedincore-shellgeometry.Themaindisad-complexmultilayercore-shellSERStagshavebeendevelopedvantageofthisapproachuptonowwasthetime-consum-recentlythroughalayer-by-layerdepositionprocedure.7-9Gener-ing(normallydays)andcomplexpreparationoftheseally,thisprocedurecanimprovethestabil
5、ityandotheropticallabeledcore-shellparticles.ThepresentedapproachcanpropertiesofSERStags.However,thisimprovementcomesatbeperformedwithinhoursonadailybase.Themainthecostofpreparationtime:obviously,themorecomplexthecharacteristicsandthestabilityofthefabricatedSERStagsstructureis,thelong
6、erpreparationtimeitneeds(usuallydaysorinextremepHvalues,highionstrength,orinorganicmore),themorechemicalsitconsumes,andtheharderthesolventisdemonstratedinthiscommunication.Further-processistohandle.6Especiallycumbersome,avitrophilic(“affinemore,theSERStagsarefunctionalizedwithanti-Salm
7、o-tosilica”)pretreatmentneedstobeperformedusuallyonsilvernellaantibodyasamodeltopresentapotentialapplica-orgoldnanoparticlestomaketheirsurfacesaffinetosilica.Merelytionofthecore-shellparticlesinbioimaging.thisprocesscantakedays;so,amorestraightforwardandsimplersynthesismethodishighlyde
8、sired
