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1、OriginalArticleInnateImmunity19(5)531–544Expressionofinnateimmunegenes,!TheAuthor(s)2013Reprintsandpermissions:proteinsandmicroRNAsinlungtissueofsagepub.co.uk/journalsPermissions.navDOI:10.1177/1753425912473668ini.sagepub.compigsinfectedexperimentallywithinfluenza
2、virus(H1N2)121,KerstinSkovgaard,SusannaCirera,DitteVasby*,2,y33AgnieszkaPodolska,SolvejØBreum,RalfDu¨rrwald,41MichaelSchlegelandPeterMHHeegaardAbstractThisstudyaimedatprovidingabetterunderstandingoftheinvolvementofinnateimmunefactors,includingmiRNA,inthelocalhostr
3、esponsetoinfluenzavirusinfection.TwentypigswerechallengedbyinfluenzaAvirussubtypeH1N2.ExpressionofmicroRNA(miRNA),mRNAandproteinswerequantifiedinlungtissueatdifferenttimepointsafterchallenge(24h,72hand14dpost-infection(p.i.).Severalgroupsofgenesweresignificantlyre
4、gulatedaccordingtotimepointandinfectionstatusincludingpatternrecognitionreceptors(TLR2,TLR3,TLR7,retinoicacid-induciblegeneI,melanomadifferentiationassociatedprotein-5),IFNandIFN-inducedgenes(IFN-b,IFN-g,IRF7,STAT1,ISG15andOASL),cytokines(IL-1b,IL-1RN,IL-6,IL-7,IL
5、-10,IL-12A,TNF-a,CCL2,CCL3andCXCL10)andseveralacutephaseproteins.Likewise,thefollowingmiRNAsweredifferentiallyexpressedinoneormoretimegroupscomparedwiththecontrolpigs:miR-15a,miR-21,miR-146,miR-206,miR-223andmiR-451.Atd1p.i.lungtissueproteinlevelsofIL-6,IL-12andIF
6、N-aweresignificantlyincreasedcomparedwiththecontrolgroup,andhaptoglobinandC-reactiveproteinweresignificantlyincreasedatd3p.i.Ourresultssuggestthat,inadditiontoawiderangeofinnateimmunefactors,miRNAsmayalsobeinvolvedincontrollingacuteinfluenzainfectioninpigs.Keyword
7、sH1N2,influenza,innate,miRNA,pigDatereceived:18October2012;revised:20November2012;accepted:11December2012IntroductionSwineinfluenzaisahighlyinfectiousrespiratorydisease1inpigscausedbyinfluenzaAvirus.SwineinfluenzaInnateImmunologyGroup,NationalVeterinaryInstitute,Tech
8、nicalposesamajorprobleminagriculturalsettingswithUniversityofDenmark,Copenhagen,Denmark2DepartmentofVeterinaryClinicalandAnimalSciences,Facultyofseveree
