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1、中国组织工程研究与临床康复第14卷第18期2010–04–30出版JournalofClinicalRehabilitativeTissueEngineeringResearchApril30,2010Vol.14,No.18自制PV液与大鼠肝脏低温保存：与UW液的对比★罗刚，罗地来，李晓征，张荣传，朱栋良Cryopreservationofratliverinself-preparedPVsolution:ComparedwithUWsolutionLuoGang,LuoDi-lai,LiXiao-zheng,ZhangRong-chuan,ZhuDong-liangAbstractDepa
2、rtmentofBACKGROUND:Atpresent,manyscholarsmakeimprovementstotheUWsolutioncomposition,aimingtofurtherexploretheHepatoliarySurgery,SecondAffiliatedprincipleoforganpreservation,improvepreservationtechniquesandtoextendthepreservationtimelimit;simplifiedcompositionHospitalofNanchangofUWsolutioncanfurtherm
3、eettheclinicaltransport,storageandeaseofuse;suitablealternativesshouldbefoundtoreduceUniversity,NanchangthecostofUWsolutiontosatisfytheneedsofthemarket.330006,JiangxiOBJECTIVE:Todiscusstheeffectivenessofself-preparedPVsolutionforpreservingrat’sliveratalowertemperature,andtoProvince,Chinacomparethere
4、sultswithUWsolution.LuoGang★,METHODS:Atotalof90Wistarratswererandomlydividedintothreegroups:UWgroup,PVgroupandnormalsaline(NS)group.Studyingformaster’sRatsineachgroupwerepreparedfornon-circulatedisolatedperfusedratlivermodels,andpreservedfor0,6,12,18,and24degree,Departmenthoursrespectively,with6anim
5、alsineachsubgroup.Thechangesofhepaticenzymology(alanineaminotransferase,aspartateofHepatoliarySurgery,Secondaminotransferaseandlactatedehydrogenase)incorrespondingtimeperiod,amountofmetabolicproductionofoxyradicalsfromAffiliatedHospitalofperfusioneffluentliquid,andthebilesecretionandchangesinhepatic
6、morphologyunderthemicroscopewereobserved.NanchangUniversity,RESULTSANDCONCLUSION:Amountsofalanineaminotransferase,aspartateaminotransferaseandlactatedehydrogenaseNanchang330006,andsuperoxidedismutaseinperfusioneffluentliquidwereclosebetweenthePVgroupandUWgroup,andthedifferencewasJiangxiProvince,Chin
7、anotsignificant(P>0.05).After6hoursofpreservation,tumornecrosisfactorαtitreinthePVfluidgroupwasgreaterthanthatinldilai868@163.comtheUWgroup(P<0.05);after12hoursofpreservation,amountofmalonaldehydeinth
