9.富氢uw液减轻大鼠肾脏低温保存期缺血损伤

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1、万方数据第19卷2期2013年3月天津医科大学学报V01．19，N。．2JournalofTianjinMedicalUniversityMar．2013塑文章编号1006—8147(2013)02—0097—04富氢UW液减轻大鼠肾脏低温保存期缺血损伤徐如彬，，杜洪印。，喻文立2，翁亦齐2。王永旺2。任恒昌1(1．天津医科大学研究生院，天津300070；2．天津市第一中心医院麻醉科，天津300192)摘要目的：研究富氢UniversityofWisconsin器官保存液(HRuw)对大鼠离体肾脏低温保存期缺血损伤的影响。方法

2、：24只大鼠随机分为3组：正常对照组(c组)、uw液保存组(uw组)和HRUW液保存组(HRuw组)，每组8只。按大鼠肾移植供肾摘取方法取下大鼠左肾。C组：肾脏不进行保存；UW组：将肾脏于4℃uw液中保存48h；HRUW组：将肾脏于4oCHRUW液中保存48h。分别于保存后观察各组大鼠肾脏组织形态学变化，进行肾小管损伤评分；检测肾脏组织中丙二醛(MDA)的含量及锰超氧化物歧化酶(Mn—SOD)、Caspase一3的活性。结果：HRuw组大鼠肾脏组织病理改变较uw组明显减轻，肾小管损伤评分明显降低(P<0．05)。与UW组相比，

3、HRUW组大鼠肾脏组织内MDA含量明显降低(P<0．01)，Mn—SOD活性升高(P<0．01)，Caspase一3活性明显降低(P℃0．叭)。结论：富氢uw液减轻大鼠肾脏低温保存期缺血损伤，其机制可能与氢气(H：)抑制低温保存肾脏氧化应激反应有关。关键词氢；肾移植；器官保存；缺血损伤；氧化应激中图分类号R617文献标志码AInfluenceofhydrogen-richUniversityofWisconsinsolutiontorenalcoldischemiainjuryinratsXURu-binl，DUHong-yi

4、n2，YUWen-li2，WENGYi—qi2，WANGYong-wan92，RENHeng-chan91(1．GraduateSchool，TianjinMedicalUniversity，Tianjin300070，China；2．DepartmentofAnesthesiology，TianjinFirstCenterHospital，Tianjin300192，China)AbstractObjective：Toevaluatetheinfluenceofhydrogen(H2)byaddingittoUniversi

5、tyofWisconsin(UW)solutiontorenalcoldis—chemiainjury．Methods：Twenty—fourWistarratswereequallyrandomizedintothreegroups：control(C)group：isolatedkidneyswithoutpreservation，UWsolution(uw)group：kidneyswerestoredinUWsolutionfor48hat4oC，andhydrogen—richUWsolution(HRUW)grou

6、p：kidneyswerestoredinHRUWsolutionfor48hat4oC．Therenalhistologicaldamageswereevaluated，whilemalondialdehyde(MDA)contents，theactivityofManganesesuperoxidedismutase(Mn—SOD)andCaspase-3inrenalsamplesweretested．Results：CornparedwithUWgroup，HRUWsolutionalleviatedtubularin

7、juryandrelievedmorphologicalrenalchanges．TissueMDAlevelsandCaspase一3activitywerealldecreasedsignificantly(P<0．01)，whiletissueMn-SODactivitywaselevated(P

8、ntcapacityofhydrogenispossi—bleprotectivemechanism．Keywordshydrogen；kidneytransplantation；organpreservation；ischemiainjury；oxidativestress