资源描述：
《2zheng68核苷二磷酸激酶mpmi.2000.13.1》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、MPMIVol.13,No.1,2000,pp.62–71.Publicationno.M-1999-1101-01R.©2000TheAmericanPhytopathologicalSocietyAnalysisofMedicagotruncatulaNoduleExpressedSequenceTagsJánosGyörgyey,1,2DanièleVaubert,1JoséI.Jiménez-Zurdo,1,3CelineCharon,1LilianeTroussard,1ÁdámKondorosi,1andÉvaKondorosi11InstitutdesSciencesVégé
2、tales,CNRS,91198Gif-sur-Yvette,France;2InstituteofPlantBiology,BiologicalResearchCenter,HungarianAcademyofSciences,H-6726Szeged,Hungary;3DepartamentodeMicrobiologíadelSueloySistemasSimbióticos,EstaciónExperimentaldelZadín,CSIC,18008Granada,SpainAccepted1October1999.Systematicsequencingofexpresseds
3、equencetags(ESTs)DistinctrootcelllayersresponddifferentlytoNodfactors.cangiveaglobalpictureoftheassemblyofgenesinvolvedInMedicagospp.,roothairsandoutercorticalcellsarein-inthedevelopmentandfunctionoforgans.Indeterminatevolvedintheinfectionprocess,forminginfectionthreadsthatnodulesrepresentingdiffe
4、rentstagesofthedevelopmentaltransportthebacteriatowardtheinnercortex.Theselatterprogramareespeciallysuitedtothestudyoforganogen-cellsareinducedfordivision,leadingtotheformationoftheesis.WiththevectorlHybriZAP,acDNAlibrarywascon-nodulemeristem.Then,cellsarrestedindivisiondifferentiatestructedfromem
5、ergingnodulesofMedicagotruncatulaintovariouscelltypes,resultinginthedevelopmentoftheinducedbySinorhizobiummeliloti.The5¢endsof389characteristicperipheralandcentralnoduletissues.cDNAclonesweresequenced,thentheseESTswereana-Medicagospp.nodulesareoftheindeterminatetype,hav-lyzedbothbysequencehomology
6、searchandbystudyingingapersistentmeristemwhere,duetothecontinuouscon-theirexpressioninrootsandnodules.Twohundredfifty-versionofmeristematiccellsintodistinctnodulecelltypes,allsixESTsexhibitedsignificantsimilaritiestocharacterizedstagesofnoduledifferentiationarerepresented.Thecentraldatabaseentries
7、and40ofthemrepresented26nodulinregionoftheindeterminatenodulesisbuiltupbythreemajorgenes,while133hadnosimilaritytosequenceswithzones:theapicalnodulemeristem,wherecellsproliferateknownfunction.Only60outofthe389cDN
