欢迎来到天天文库
浏览记录
ID:38627406
大小:465.01 KB
页数:10页
时间:2019-06-16
《《人工合成基因组》PPT课件》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、人工生命—artificiallife2007年6月美国科学家克雷格•文特尔和他领导的研究小组宣布,他们首次实现了完整的基因组在物种间的移植,这一成功为首个「人造生命」的降生奏响了序曲。文特尔说,这次成功让他向着制造出首个「人造生命」又迈进一步,他将在几个月内利用人工合成的基因组展开类似的移植试验,实现科研史上零的突破。如果试验成功,文特尔就能宣布他造出全球第一个「合成生命」形式。2010年5月20日J.CraigVenterInstitute(JCVI),anot-for-profitgenomicresearchorganization
2、,publishedresultstodaydescribingthesuccessfulconstructionofthefirstself-replicating,syntheticbacterialcell.Theteamsynthesizedthe1.08millionbasepair(1080kb)chromosomeofamodifiedMycoplasmamycoidesgenome.ThesyntheticcelliscalledMycoplasmamycoidesJCVI-syn1.0andistheproofofprin
3、ciplethatgenomescanbedesignedinthecomputer,chemicallymadeinthelaboratoryandtransplantedintoarecipientcelltoproduceanewself-replicatingcellcontrolledonlybythesyntheticgenome.ThisresearchwillbepublishedbyDanielGibsonetalintheMay20theditionofScienceExpressandwillappearinanupc
4、omingprintissueofScience.Genometransplantationinbacteria:changingonespeciestoanother天然完整基因组种间转移:Asasteptowardpropagationofsyntheticgenomes,wecompletelyreplacedthegenomeofabacterialcellwithonefromanotherspeciesbytransplantingawholegenomeasnakedDNA.IntactgenomicDNAfromMycopl
5、asmamycoides(蕈状支原体)largecolony(LC),virtuallyfreeofprotein,wastransplantedintoMycoplasmacapricolum(山羊支原体)cellsbypolyethyleneglycol(PEG)-mediatedtransformation.Cellsselectedfortetracyclineresistance,carriedbytheM.mycoidesLCchromosome,containthecompletedonorgenomeandarefreeof
6、detectablerecipientgenomicsequences.ThesecellsthatresultfromgenometransplantationarephenotypicallyidenticaltotheM.mycoidesLCdonorstrainasjudgedbyseveralcriteria.---Science.2007Aug3;317:632-8合成基因组---Science论文Science2July2010:Vol.329.no.5987,pp.52-56CreationofaBacterialCellC
7、ontrolledbyaChemicallySynthesizedGenomeDanielG.Gibson,1JohnI.GlassetalWereportthedesign,synthesis,andassemblyofthe1.08–mega–basepairMycoplasmamycoidesJCVI-syn1.0genomestartingfromdigitizedgenomesequenceinformationanditstransplantationintoaM.capricolumrecipientcelltocreaten
8、ewM.mycoidescellsthatarecontrolledonlybythesyntheticchromosome.TheonlyDNAinthecellsisthed
此文档下载收益归作者所有