洋葱多糖的提取工艺改进

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1、安徽农业科学,JournalofAnhuiAgr.iSc.i2010,38(10):5113-5114,5124责任编辑高晓余责任校对况玲玲洋葱多糖的提取工艺改进黄爱民,马林,杨华,廖丹葵,黄祖强(广西大学化学化工学院,广西南宁530004)摘要[目的]改进洋葱多糖的提取工艺。[方法]采用热水浸提法提取洋葱水溶性多糖,采用正交试验考察了提取时间、提取温度和固液比对洋葱多糖得率的影响;并在此基础上考察了超声波外场强化对洋葱多糖提取的影响。[结果]热水浸提法提取洋葱水溶性多糖的最佳工艺为:提取时间为4h,提3次共计12h,提取温度60e,料液比为1B30,此时洋葱多糖得率为4.034%。在上述

2、最佳提取条件下,超声波外场强化处理频率为59kHz,功率为112W,超声时间为30min,多糖得率可达6.05%,提取时间减少4h。超声波外场强化处理有助于洋葱多糖提取效率的提高。[结论]该研究为洋葱多糖提取工艺的改进提供了理论依据。关键词洋葱多糖;正交试验;提取;外场强化中图分类号R284.2文献标识码A文章编号0517-6611(2010)10-05113-02ResearchonExtractionMethodsofPolysaccharidefromOnionHUANGA-iminetal(SchoolofChemistryandChemicalEngineering,Guangx

3、iUniversity,Nanning,Guangxi530004)Abstract[Objective]Tomiprovetheextractiontechnologyofpolysaccharidesfromonion.[Method]Theextractionmethodsofpolysaccha-ridefromonionwerestudiedbyorthogonalexpermientswithhotwaterextractionmethod.Theeffectfactorsincludingextractiontmie,tempera-tureandtheratioofsoli

4、d-liquidontheextractionratewereinvestigated.Furthermore,theultrasonicassistedextractionmethodswasinvestiga-tedtopromotetheextractionrate.[Result]Theoptmiumextractingprocessofpolysaccharidefromonionwithhotwaterextractionmethodwasshowedasfollowing:extracting3tmies,4heverytmie,extractingtemperatureat

5、60e,theratioofsolid-liquidat1B30,andtheextractionrateofpolysaccharidewasupto4.034%.Usingtheoptmiumextractingprocessabove,ultrasonicfrequencyat59kHz,powerat112Wandextractiontmiein30minutes,theextractionrateofpolysaccharidewasupto6.052%withtheseultrasonicoutfieldintensifyingconditions.Theextractiont

6、mieoftheultrasonicassistedextractionmethodswasalsodecreasedby4h.Outfieldintensifyingultrasoundtreatmentscouldin-creaseintheefficiencyofextractingpolysaccharidefromonion.[Conclusion]Theseresultsprovidedthetheoreticbasisfortheextractiontech-nologyofpolysaccharidesfromonion.KeywordsOnionpolysaccharid

7、e;Extraction;Orthogonaltes;tOutfieldintensifying洋葱(AIliumcepaL.)为百合科(Liliaceae)葱属(Ailium)1.2水提醇沉法提取洋葱多糖植物,具有消炎抑菌、活血化淤、防癌抗癌、利尿、降血糖以及1.2.1原料预处理。准确称取20g洋葱粉置于500ml圆底[1]预防心血管疾病等功效。洋葱的化学成分主要为硫化物、烧瓶中,加入400ml95%乙醇,在85e水浴