慢性乙型肝炎患者肝组织中HBV_CCCDNA定量与病情相关性分析

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1、山东医药2010年第50卷第7期慢性乙型肝炎患者肝组织中HBVcccDNA定量与病情的相关性分析盛慧萍，杨岩’。赖雅芳。刘娅，张栩，秦琨(宁夏医科大学附属医院，银川750004)摘要：目的探讨肝组织HBVcccDNA定量对慢性乙型肝炎(乙肝)患者病情的影响。方法应用FQ—PRC法检测42例乙肝患者肝组织HBVcccDNA、肝组织和血清HBV—DNA水平，同时对肝组织行常规病理染色判断肝组织炎症及纤维化程度；SP法检测肝组织中I-1BsAg、HBcAg表达，化学发光法检测HBV标志物。分析肝组织HBVcccDNA与组织和血清HBV．D

2、NA、肝细胞内HBsAg、HBcAg水平及肝脏炎症、纤维化程度的关系。结果肝组织HBVcccDNA定量与组织及血清HBV—DNA定量呈正相关(r=0．807，P<0．001；r=0．627，P<0．001)；与肝组织炎症活动度及纤维化程度无明显相关性；肝组织HBVcccDNA定量与肝细胞内HBsAg表达无明显相关性，而与HB—eAg表达呈正相关(r=0．486，P<0．05)。结论检测肝组织内HBVeccDNA可更精确反映HBV复制程度；对乙肝诊断、抗病毒治疗及选择停药时机具有重要价值。肝组织HBVcccDNA与血清HBV-DNA定

3、量及HBcAg联合检测可指导抗病毒治疗。关键词：慢性乙型肝炎；共价闭合环状DNA；乙肝病毒表面抗原；乙肝病毒核心抗原中图分类号：R512．62文献标志码：A文章编号：1002-266X(2010)07-0012-03AnalysisofthecorrelationbetweenthequantitiesofhepatitisBviruscovalentlyclosedcircularDNAandconditioninchronichepatitisBpatientsSHENGHui-ping，加Yan，，扣，，ZHANGXu，Qmr

4、ing(liatedHospitalofNingxiaMedicalUniversity，Yinchuan710004，P．R．China)Abstract：Obj~'tlveToinvestigatetheefectofHBVcccDNAquanfitationontheconditionofchronichepatitisB(CHB)patients．MethodsThequantityofHBVeccDNAandHBVDNAinthehepatoeytesandHBVDNAintheserunl0f42patientswith

5、CHBweredetectedbyreal—timefluorescencequantitativePCR(FQ．PCR)．Theinflammationandfibro-sisdegreewereestimatedbypathologicalstaining．TheexpressionofHBsAg．HBcAginthehepatocyteswereidentifiedbySPmethod．CorrelationsbetweentheHBVcccDNAquantitationandotherindexeswereanalyzed．

6、ResultsTheHBVCCCDNAquantitationintheEvertissuewerepositivelycorrelatedtotheHBVDNAlevehinthelivertissueandserafr；0．807，P<0．001．r=0．627，P<0．001)，andwerenotcorrelatedtothedegreeofhverinflammationandfibrosis．TheHBVeecDNAquantitationinthelivertissuewerenotcorrelatedtotheexp

7、ressionofHBsAg，butpositivelycorrelatedtotheexpressonofHBcAg(r=0．486，P<0．05)．CondusionsThequantitationofHBVcccDNAcallreflectthereplicationofHBVmoreaccurately，andplaysimportantroleinthediagnosis，antiviraltreatmentandwithdrawaltimeselectionofCHB．Itismorereliabletotaketheq

8、uantificationofhepatictissueHBVCCCDNAcombiningtheHBVDNAintheser1．1／／landtheexpressiondegreeofHBcAgasantivirustherapeu