种植体表面粗糙度对成骨细胞增殖及ALP含量的影响_范震

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1、口腔颌面外科杂志2009年4月第19卷第2期·128·JournalofOralandMaxillofacialSurgeryVol．19No．2April,2009·口腔种植园地·种植体表面粗糙度对成骨细胞增殖及ALP含量的影响范震121，贾爽，苏剑生（1.同济大学附属口腔医院修复科，上海200072；2.上海市口腔病防治院修复科，上海200001）[摘要]目的：何种粗糙度的种植体能产生最快、最强的骨结合尚有争议，本研究将成骨细胞接种到不同粗糙度的钛盘表面，观察成骨细胞生长、增殖及碱性磷酸酶（alkalinephosphatase,ALP）分泌情况，探讨最适合的种植体表面粗糙

2、度范围。方法：纯钛圆盘试件48个，分为4组，前三组喷砂加双重酸蚀粗化处理，获得表面粗糙度为（1.00±0.20）、（1.67±0.08）、（2.40±0.20）μm的三组试件。未处理的试件粗糙度为（0.12±0.03）μm，作为对照组。将成骨细胞接种到4组试件表面，观察成骨细胞形态、增殖以及ALP含量的变化，并作统计分析。结果：成骨细胞在粗糙度1.00μm的钛盘表面呈单层生长；粗糙度1.67μm的钛盘表面细胞呈立方形，有较多伪足，细胞数量及ALP含量最多；粗糙度2.40μm的试件细胞生长不规则，细胞数量及ALP含量最低。结论：粗糙的种植体表面比光滑表面更有利于成骨细胞的黏附、增

3、殖及表达。其中粗糙度为1.00～2.40μm时成骨细胞增殖和表达最显著。[关键词]成骨细胞；表面粗糙度；碱性磷酸酶[中图分类号]Q813[文献标识码]A[文章编号]1005-4979（2009）02-0128-04MicroroughImplantSurfaceandOsteoblastAdhesion121FANZhen,JIAShuang,SUJian-sheng(1.DepartmentofProsthodontics,HospitalofStomatology,TongjiUniversity,Shanghai200072;2.DepartmentofProsthodo

4、ntic,ShanghaiStomatologicalDiseaseCenter,Shanghai200001,China)[Abstract]Objective:Tostudywhetherthevariationofimplantsurfaceroughnesscouldinterferetheosseointegrationofdentalimplants.Methods:Aftercoarsenedbysandblastingwithcarborundumgranulaandbymixingliquidofhydrochloricacidandsulphuricacid

5、,3differentmicropatternedsurfacewerecreatedonthetitaniumimplants:low-roughness（0.5～1μm）、mid-roughness(1～2μm)andhigh-roughness（2～3μm）;theuntreatedspecimenwasusedascontrol.Osteoblastswereintroducedontothesesurfaces,andtheirmorphology,proliferationandsecretionofalkalinephosphatase(ALP)wereevalu

6、ated.Results:Osteoblastsadheredtightlytothetitaniumsurfaceandshowedmonolayeronthelow-roughtitaniumspecimen.Cellswereastero-shapedorfusiformshapedandstretchedoutmanypseudopodsinthemedium-roughgroupwhilecellsinthehighroughnessgroupgrewmoreirregularly.Cellscultivatedonthemedium-roughnessgroupgr

7、ewmorequicklythanontheothertwogroups,andcontentofALPinthisgroupwasthehighest.Whilethehigh-roughnessgrouphadtheleastnumberincellandALPsecretion.Conclusion:Roughsurfaceofimplantdopromotetheadherence,proliferationandALPsecretionofosteoblasts.Roughness