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1、辽宁中医药学报十五卷护骨胶囊对成骨细胞增殖、分化影响李宝红1，吴劲东2，赵文昌1，宋丽军1(1.广东医学院药学院广东东莞523808；广东医学院药理学教研室，广东东莞523808)摘要：目的：探讨护骨胶囊对体外培养的大鼠成骨细胞增殖、分化的影响。方法：采用多次酶消化法分离出大鼠颅盖骨的成骨细胞,采用MTT法和碱性磷酸酶法分别检测护骨胶囊对成骨细胞增殖和分化的作用;采用SYBRgreenI嵌合的RT-PCR方法检测Runx2mRNA表达水平。结果：护骨胶囊在浓度0.025~2.5μg/mL对成骨细胞有促增殖的作用,护骨胶囊在浓度0.025~25μg/mL对碱性磷酸酶的活性均有提高
2、的作用,护骨胶囊在浓度25μg/mL下,对Runx2mRNA水平有上调的作用。结论：护骨胶囊对成骨细胞有促增殖、促分化的作用,可能与上调Runx2mRNA水平有关。关键词：护骨胶囊；成骨细胞；碱性磷酸酶；Runx2中图分类号：R283文献标志码：A文章编号：1673-842X（2013）10-0024-03EffectofCapsuleonProliferationandDifferentiationofPrimaryOsteoblastsinVitroLIBaohong,WUJindong,ZHAOWenchang,SONGLjiun(1.CollegeofPharmacy,
3、GuangdongMedicalCollege,Dongguan523808,GuangdongChina;2.DepartmentofPharmacology,GuangdongMedicalCollege,Dongguan523808Guangdong,China)Abstract:Objective:ToinvestigatetheeffectofHuguCapsuleonproliferationanddifferentiationinprimaryosteoblasts.Methods:Bonecellswereobtainedbyenzymedigestionfro
4、mthesegregatedneonatalSDratskull.TheeffectsofHuguCapsuleontheproliferationanddifferentiationofratosteoblastswereevaluatedbytheMTTmethod,measuringtheavtivityofalkalinephosphatase,respectively.Runx2expressionwasanalyzedbyreal-timefluorescentquantitativereversetranscriptasepolymerasechainreacti
5、on(qRT-PCR)usingSYBAGreenI.Results:Withtheconcentrationof0.025-2.5μg/mL,HuguCapsulehadsignificanteffectontheROB’sproliferation.TheALPactivitywassignificantlyincreasedattheconcentrationfrom0.025μg/mLto25μg/mL.Withtheconcentrationof25μg/mL,HuguCapsulecanincreasetheRunx2MrnalocatedintheTomb,Don
6、gShenJiabang,deferthenextdayfocusedontheassassination.Linping,Zhejiang,1ofwhichliquorwinemasters(WuzhensaidinformationisCarpenter),whogotAfewbayonets,duetomissedfatal,whennightcame辽宁中医药学报十五卷level.Conclusions:HuguCapsulecanstimulatetheproliferationanddifferentiationofosteoblastsinvitro,andthe
7、reasonmaybeconnectedwiththeupregulationofRunx2mRNAlevel.Keywords:HuguCapsule;osteoblasts;alkalinephosphate;Runx2护骨胶囊是由淫羊藿、制何首乌、巴戟天、骨碎补、龟甲、山药、熟地黄、当归、杜仲、续断等组成的中药复方新药，临床试验表明其具有较好的治疗原发性骨质疏松症和绝经后骨质疏松症的作用[1-2]。但目前对其抗骨质疏松的作用机制尚未进行深入研究，本项目通过探讨护骨胶囊对大鼠原代成骨细胞
