资源描述：
《重复经颅磁刺激对帕金森模型小鼠纹状体VMAT_2和SYN表达的影响》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、第30卷第4期第三军医大学学报Vol.30,No.43222008年2月ACTAACADEMIAEMEDICINAEMILITARISTERTIAEFeb.2008论著文章编号:100025404(2008)0420322204重复经颅磁刺激对帕金森模型小鼠纹状体VMAT22和SYN表达的影响王全懂,董巧云,顾平,王彦永,刘静,王铭维(河北医科大学第一医院神经内科,河北省脑老化与认知神经科学重点实验室,石家庄050017)摘要:目的探讨重复经颅磁刺激(repetitivetranscranialmagn
2、etic,rTMS)对帕金森病(Parkinson’sdisease,PD)模型鼠纹状体多巴胺(dopamine,DA)含量及Ⅱ型单胺囊泡转运体(vesicularmonoaminetransporter22,VMAT22)和突触体素(synaptophysin,SYN)表达的影响。方法65只雄性C57BL/6J小鼠随机分为:对照组、PD模型组、假磁刺激组、rTMS21-1组、rTMS22组,每组13只。小鼠皮下注射MPTP(15mg·kg,每小时注射1次,注射4次)复制帕金森病模型,重复经颅磁刺激干预
3、,利用高效液相色谱2电化学(HPLC2ECD)测定纹状体DA含量;免疫组织化学染色检测VMAT22和SYN的表达变化,并借助图像分析系统对其进行定量分析。结果rTMS21组和rTMS22组纹状体DA含量较对照组明显减少(P<0101),但显著高于PD模型组和假磁刺激组(P<0101);rTMS21组和rTMS22组纹状体VMAT22和SYN表达均明显少于对照组且显著高于PD模型组和假磁刺激组,其免疫反应产物校正光密度值(COD)均显著低于对照组(P<0105),并显著高于PD组和假磁刺激组(P<0105
4、),TMS21组和rTMS22组间无显著差异。结论rTMS可诱导PD鼠纹状体区DA释放增加,并使VMAT22和SYN表达上调。关键词:重复经颅磁刺激;多巴胺;纹状体;SYN;VMAT22中图法分类号:R338.2;R454.1;R742.5文献标识码:AEffectofrepetitivetranscranialmagneticstimulationonvesicularmonoaminetrans2porter22andsynaptophysinexpressionsinstriatumofParki
5、nson’sdiseasemodelmiceWANGQuan2dong,DONGQiao2yun,GUPing,WANGYan2yong,LIUJing,WANGMing2wei(DepartmentofNeurolo2gy,TheFirstHospitalofHebeiMedicalUniversity,BrainAgingandCognitiveNeuroscienceLaboratoryofHebeiProvince,Shijiazhuang050017,China)Abstract:Object
6、iveToinvestigatetheeffectofrepetitivetranscranialmagneticstimulation(rTMS)onthecontentsofDopamineandvesicularmonoaminetransporter22(VMAT22)andsynaptophysin(SYN)expres2sionsinstriatumofParkinson’sdisease(PD)modelmice.MethodsSixty2fivemaleC57BL/6Jmicewerer
7、andomlydividedinto5experimentalgroups:controlgroup,PDmodelgroup,shamrTMSgroup,rTMS21groupexposedto110%motionthreshold,rTMS22groupexposedto130%motionthreshold,with13miceineachgroup.ThemodelofParkinsion’sdiseaseofC57BL/6Jmiceweremadebythesubcutaneousinject
8、ionofMPTPsolutionandthenweretreatedwithrTMSfor14days.ThecontentsofDopamineinthestriatumweremeasuredbyhighperformanceliquidchromatography(HPLC2ECD).TheVMAT22andSYNexpressionsinstriatumweredetectedbyimmunohistochemistryandan
