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1、天然产物研究与开发NatProdResDev2009,21:8222825,839文章编号:100126880(2009)0520822205高速逆流色谱法分离凤尾草中的芹菜素和木犀草素*欧阳丹薇,孔德云,许海燕,倪翔,陈佳,杨培明上海医药工业研究院中药室,上海200040摘要:建立了高速逆流色谱分离纯化芹菜素和木犀草素的方法。两相溶剂系统为氯仿2甲醇2水(4B3B2),上相为固定相,下相为流动相进行洗脱。从100mg粗提物中一步分离得到17.0mg芹菜素和22.5mg木犀草素,经113高效液相色
2、谱分析,纯度分别为92%和96%。其化学结构由H和CNMR鉴定。关键词:高速逆流色谱;凤尾草;黄酮;芹菜素;木犀草素中图分类号:R284.2;Q946.91文献标识码:AIsolationandPurificationofApigeninandLuteolinfromPterismultifidaPoir.byHigh2speedCounter2currentChromatography*OUYANGDan2we,iKONGDe2yun,XUHai2yan,NIXiang,CHENJia,YANG
3、Pei2mingDepartmentofChineseTraditionalMedicine,ShanghaiInstituteofPharmaceuticalIndustry,Shanghai200040,ChinaAbstract:High2speedcounter2currentchromatography(HSCCC)wasappliedtoisolateandpurifybioactivecompoundsfromPterismultifidaPoir.Twoflavonoidsapig
4、eninandluteolinweresuccessfullyisolatedandpurifiedbyusingtwo2phasesolventsystem,whichwascomposedofchloroform2methanol2water(4B3B2,v/v/v)inheadtotailelutionmode.Consequently,17.0mgofapigeninand22.5mgofluteolinwereobtainedfrom100mgcrudesamplewithpuritie
5、sof11392%and96%,respectively,asdeterminedbyHPLC.ThechemicalstructureswereidentifiedbyHandCNMR.Keywords:high2speedcounter2currentchromatography;PterismultifidaPoir.;flavonoids;apigenin;luteolinatedwiththesolidsupports.Inaddition,itoperatesun2Introducti
6、onderverygentleconditionsandallowsnon2destructive[6]Fengweicao,thedriedherbofPterismultifidaPoir.,isseparationofcompounds.Asaresul,tthistechnologyatraditionalChinesemedicine.Itcouldbeeasilyfoundhasrecentlygainedincreasinglyimportanceinthesep2andwidely
7、usedinChina.Ithastheefficacyofenteri2arationofnaturallyoccurringproducts,andhasbeentis,hepatitis,bacterialdysentery,haematemesis,haema2successfullyappliedtotheseparationofanumberof[1,2][7210]turia,tonsillitis,parotitisandeczema.Theeffectivenaturalprod
8、ucts.Althoughapigeninandluteolincomponentsofthisherbareflavonoids,sesquiterpe2havebeenpurifiedfromP.multifidaPoir.,noreport[3,4]noidsandditerpenoids.Pharmacologicaltestre2hasbeenrevealedconcerningusageofHSCCCforthevealedthattheflavonoidsapigen
