Phenyl Sepharose High Performance

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1、GEHealthcareDataFile18-1172-87ACHydrophobicinteractionchromatographyPhenylSepharoseHighPerformanceButylSepharoseHighPerformancePhenylSepharose™HighPerformanceandButylSepharoseHighPerformancearehydrophobicinteractionchromatography(HIC)media.Theirperformanceischaracte

2、rizedby:•High-resolution,high-capacityseparationswithhighrecovery•Reliableandreproducible•HighchemicalstabilityforeffectiveCIPandsanitization•AvailableinlaboratoryandBioProcess™scalequantities•EasytoscaleupThemediaoccupyakeypositionintheintermediateandfinalpurificat

3、ionstagesofawidevarietyofproteinsandFig1.PhenylSepharoseHighPerformanceandButylSepharoseHighPerformance,availableinavarietyofformats,allowhigh-resolution,high-peptides.Theiravailabilityinprepackedcolumns,suchcapacitypurificationofbiomolecules.as,HiLoad™andHiTrap™plu

4、slaboratoryandBioProcesspacksizesmakespurificationatallscalessimpleandconvenient(Fig1).Hydrophobicinteractiongelfiltration.Forexample,HICmakesanideal“nextstep”chromatographywhenpurifyingmaterialthathasbeenprecipitatedwithHydrophobicinteractionchromatographyseparates

5、andammoniumsulfateorelutedinhighsaltconcentrationspurifiesbiomoleculesbasedondifferencesintheirsurfaceduringionexchange.hydrophobicity.ThetechniqueisversatileandoffersHICisusuallyperformedinmoderatetohighconcentrationsspecificselectivity.Manyproteinsandpeptides,aswe

6、llasofsaltsinthestartbuffer,whichpromotesbindingandotherhydrophobicbiomoleculeshavesufficientnumbershelpstostabilizetheproteinstructure.Theboundmoleculeofexposedhydrophobicgroupstoallowinteractionwithiselutedbydecreasingthesaltconcentrationinalinearhydrophobicligand

7、scoupledtochromatographicmatrices.orstepwisemanner.ContinuousgradientelutionismostComparedwithReversedPhaseChromatography(RPC)frequentlyusedwhenhighresolutionisneededandadsorbents,HICmediadisplaymilderelutionconditionsstepwisegradientelutionisrecommendedforsampleand

8、consequentlybetterretentionofbiologicalactivitypreparationandconcentration.Severalfactorsinfluenceafterseparation.HICiswellsuitedforuseint