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1、第24卷第5期高校化学工程学报No.5Vol.242010年10月JournalofChemicalEngineeringofChineseUniversitiesOct.2010文章编号：1003-9015(2010)05-0897-06响应面法优化超临界CO2提取茶籽多糖的工艺研究李博,屠幼英,梅鑫,蔡晓红,王奕,李伟(浙江大学茶学系,浙江杭州310029)摘要：茶多糖具有多种重要的生物活性。本研究采用响应面法优化茶籽多糖的超临界CO2提取工艺，运用小星点设计考察萃取时间(30~150min)、压力(15~45MPa)、温度(40
2、~80℃)及夹带剂乙醇浓度(50%~90%)四因素对茶籽多糖得率的影响。响应面分析表明，茶籽多糖得率与四因素关系符合二次模型。时间和夹带剂乙醇浓度的一次项和二次项、压力的二次项以及时间和压力的交互作用对茶籽多糖得率具有显著影响。该二次多项式模型用后退法进行逐步回归得到简化模型，依据简化后的模型在各因素设定范围内获得最佳提取工艺为：时间150min，压力45MPa，温度60℃，夹带剂乙醇浓度为65%。该条件下进行三次重复试验，茶籽多糖的实际平均得率为13.23%，与预测值13.96%无显著差异。关键词：超临界CO2；茶籽；多糖；响应面分
3、析；小星点设计中图分类号：TQ028.32文献标识码：AOptimizationofSupercriticalCO2ExtractionProcessofthePolysaccharidefromTeaSeedbyResponseSurfaceMethodologyLIBo,TUYou-ying,MEIXin,CAIXiao-hong,WANGYi,LIWei(DepartmentofTeaScience,ZhejiangUniversity,Hangzhou310029,China)Abstract:Teapolysaccharid
4、ehasvariousimportantbiologicalactivities.ThesupercriticalCO2extractionprocesswiththeethanolaqueoussolutionassolventmodifierwasusedtoextractthepolysaccharidefromteaseed,andtheresponsesurfacemethodology(RSM)wasusedtooptimizetheprocessparameters.Fourprocessparameters,extra
5、ctiontime(30~150min),pressure(15~45MPa),temperature(40~80℃)andtheethanolcontentinmodifier(50%~90%)wereselectedasfourindependentvariables.Asmallcentralcompositedesign(smallCCD)wasusedtostudytheeffectsoftheabovefourindependentvariablesontheyieldoftheextractedteaseedpolysa
6、ccharide.Thequadraticformulawasobtainedbyresponsesurfaceanalysisbetweentheyieldoftheextractedteaseedpolysaccharideandthefourparameters.Thelinearandquadratictermoftimeandethanolconcentration,thequadratictermofpressureandtheinteractionbetweentimeandpressurehavesignificant
7、effectsontheyieldofteaseedpolysaccharide.Thesecond-orderpolynomialmodelwassimplifiedbyusingabackwardstepwisemethod,andthentheoptimumparameterswithintheexperimentalrangesweredeterminedtobeat150mim,45MPa,60℃andwith65%ethanolaqueoussolution.Undertheseconditions,theaveragev
8、alueoftheactualyieldoftheteaseedpolysaccharideinthreereplicatedexperimentsis13.23%,andthisvalueisnotsignifican
