儿童流感嗜血杆菌氨苄青霉素耐药基因的研究

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1、亘医学院学报2008年第48卷第6期ActaAcademiaeMedicinaeJiangxi2008，Vol48，No6儿童流感嗜血杆菌氨苄青霉素耐药基因的研究陈强，余晓君，周红平，刘建梅。，杨智辉，段荣，张小玲，章高平，李岚，朱晓华(江西省儿童医院a．呼吸内科；b．检验科，南昌330006)捅姜：目的研究儿童流感嗜血杆菌(HI)对氨苄青霉素的耐药状况，并从分子生物学的角度研究其耐药机制。方法对儿童痰标本及咽拭子中分离的55株HI菌株进行p一内酰胺酶检测，PCR扩增p一内酰胺酶基因TEM一1、ROB一1的编码基因，并进行核酸序列测定与分析。结果分离的55株HI有17株产生8一内

2、酰胺酶，并对氨苄青霉素耐药，耐药率3O．9。未发现7-内酰胺酶阴性而对氨苄青霉素耐药的菌株。PCR法检测产酶HI中7-内酰胺酶基因TEM一1阳性率为100，不产酶H1中无TEM一1阳性者。在32株菌株(17株产酶菌和15株不产酶菌)中未检测到ROBq基因。结论流感嗜血杆菌是急性下呼吸道感染的主要病原体之一，对氨苄青霉素的耐药率较高。流感嗜血杆菌对氨苄青霉索的耐药主要由质粒介导的TEM-1型G一内酰胺酶所致。关键词：儿童；流感嗜血杆菌；氨苄青霉素；内酰胺酶；耐药基因中图分类号：R511．6文献标识码：A文章编号：1000—2294(2008)06—0060—03StudyofAmp

3、icillinResistantGeneinHaemophilusInfluenzaeandinChildrenCHENQiang，YUXiao-jun，ZHOUHong-ping，LIUJian—mei。YANGZhi—hui，DUANRong，ZHANGXiao-ling，ZHANGGao-ping“，LILan，ZHUXiao-hua。(a．DepartmentofRespiratoryMedicine；b．DepartmentofClinicalLaboratories，JiangxiProvinceChildren’SHospital，Nanchang330006，Ch

4、ina)ABSTRACT：ObjectiveTostudythesituationofampicillinresistanceofhaemophilusinfluenzae(HI)andtoexploretheresistantmanehnismofHIbvpolymerasechainreaction．~VlethodsFif—ty—fivestrainsofHIisolatedfromsputumspecimensandthroatswabsofchildrenwerecollectedtodetectbeta—lactamase．TEM一1andR()B一1beta—lac

5、tamasegenesweredetectedbypolymerasechainreaction(PCR)andnucleotidesequencing．ResultsBeta—lactamasewasproducedin17strainsoutof55strainofHI．Moreoverithasampicillinresistant．Therateofampicillinresist—ancewas30．9％inHI．Nobeta-lactamase—negativeampicillinresistantH1wasidentified．100ofthebeta—lactam

6、ase—positivestrainsgaveapositiveresultwithspecificprimersfortheTEM一1gene．NonestrainwasdetectedfortheROB一1genewithin32strainscilstrainsinbeta—lactamase—positivestrainsand15strainsinbata—lactmase—negarivestrains．ConclusionHIisolatedfromchildrenwithlowerrespiratorytractinfectionwouldbeamajorpath

7、ogen，andampicillinresistantinH1wasratherhigh．TheresistancemechanismofampicillinresistantH1wascausedbybeta—iactamase，mainlyplasmidmediatedTEM一1typeenzyme．KEYWORDS：child；haemophilusinfluenzae；ampicillin；beta-lactamase；resistantgene流感嗜血杆菌(haemop