孕期尼古丁暴露致子代宫内发育迟缓大鼠骨关节炎易感

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1、第44卷第6期第648页华中科技大学学报(医学版)V01．44NO．6P．6482O15年12月ActaMedUnivSciTeehnolHuazhongDec．2O15孕期尼古丁暴露致子代宫内发育迟缓大鼠骨关节炎易感*上官杨帆，汪晖，谭杨，王林龙，曾一繁，张先荣。，陈廖斌武汉大学中南医院骨科基础医学院药理学系。基础医学院生理学系，武汉430071摘要：目的观察孕期尼古丁暴露(prenatalnicotineexposure，PNE)所致宫内发育迟缓(intrauterinegrowthrestric—tion，IUGR)子代大鼠老年期骨关节炎(osteoarthritis，O

2、A)的易感性及机制。方法于大鼠孕11d起注射尼古丁[2mg／(kg·d)]至分娩。正常对照组及PNE子代IUGR大鼠断奶后至6月龄给予高脂饮食，16．5月龄时以长距离跑步6周诱发0A发生。取大鼠膝关节标本切片进行改良Mankin’S评分及免疫组化检测分析。结果与正常对照组相比，PNE组大鼠出现典型OA症状，如软骨基质浅染、软骨纤维化，Mankin评分显著升高(P

3、炎；胰岛素样生长因子一1中图分类号：R684．3D0I：10．3870／j．issn．1672—0741．2015．06．006PrenatalNicotineExposureEnhancestheSusceptibilityofOsteoarthritisinRatOffspringwithIUGRShangguanYangfan，WangHui，TanYangetalDepartmentofOrthopedics，ZhongnanHospitalDepartmentofPharmacology，SchoolofBasicMedicalSciences，WuhanUniver

4、sity，Wuhan430071，ChinaAbstract0bjectiveToinvestigatethesusceptibilityofosteoarthritis(OA)andtheunderlyingmechanismintheratoff—springwithintrauterinegrowthretardation(IUGR．)inducedbyprenatalnicotineexposure(PNE)．MethodsRatsweresubcuta—neouslyinjectedwithnicotine[2mg／(kg·d)fromgestationalday11

5、untildelivery．Theoffspringratswerefedonhigh—fatdietfromtheablactationto6monthsoldinbothnormalcontro1andPNEgroups．Forcedrunningstimulationwasadministratedtooffspringratsat16．5monthsoldfor6weekstoinduceOA．ThekneeiointswereremovedfortheModifiedMankin’Sscoringandimmunohistochemistryanalysis．Resu

6、lts0ffspringratsinPNEgroupshowedtypicalOAfeatures．suchasweakstainingofthearticularcartilagematrix，increasedfibrosisofthearticularcartilage．andincreasedMankin’Sscores．Theimmunohistochem—icalassayshowedsignificantlyreducedexpressionofIGF1inPNEgroupwhencomparedwiththenormalcontrolgroup(P

7、ConclusionPNEincreasesthesusceptibilitytOsenileOAinoffspringrats。whichmaybeassociatedwiththelOWex—pressionofIGF-1inthearticularcartilage．Keywordsnicotine；intrauterinegrowthretardation；osteoarthritis；insulinlikegrowthfactor-1骨关节炎(osteoarthritis，OA)是