O2、ACC和CO2对番木瓜ACC氧化酶活性的影响

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1、万方数据Acm册q印如s如姗函d蜘如n200I，27(s)：387～392387Ef托ctof02，ACCandC02ConcentrationontheActiVityofPartiallyPuri6edACCoxidasefromPapayasONGso“g—Quanl，2”wing—KinYip2(17heK“kbo川o，y矿＆月en螂7Ⅲw旷Ⅳ【『lmv矿E血cⅡfm“，sckDf矿L沿＆正，"es，刀【m够^曲№‰咿，Gu北胆hou51【】275；20印“n删Ⅲ订日ofⅡHy，om础5町o，日m29KDr蜡，P。UikmR。耐，Hong

2、Kong)Abstract：l—Aminocyclopmpanc一1一carboxlicacid(ACC)isox讯ized协fonnethvknebvACCoxidaseThep州i“pu打6cationofACCoxidasefromhiLpeels0fp印8yaa∞deffecfsofsubstrales(“ygⅫandACC)．cofacLors(CO，andFe“)andinhibitors(Co¨and眦一aminoisobutvricacid)onmoz￡roethvI—encp1吣ductionratewereinve札i冀8te

3、d．ACCo妇dasehasbee”purined19．5—foldbyusi”gDEAE—Sepharoseandne”y1一S。phamsecolumns．11heK。valuebyACCoxidascfor02ine山ylenepmductionva^edgrea【lyde—pendingonIheACCconcen岫don，anddecreascd¨i【}IincreaseoI_ACClevels．TheKvalueforACCal—sodeclinedasox坩肆nconc咖frationinc肿8sed．Theenzvmeacllvi

4、”wasdr日m“calhincreasedbvCO，，andtheK。vaIuefor0，andforACCincreasedwithincreaseofCO，concentra“onEnzvmc8ctivitieswercohvioudvincreasedbvFe¨andsignincandvinhibitedb～C02_；andFe¨couldanl日gonizefl】ejIlhmiiowroJeofC02。’rhesekineficdataaIeconsistentwith血evjew出afIheca妇lv￡icf日leofACCo秭da

5、sefollowsanordeledbindi“gmechanismlnwhichACCoxidasebinds耵rsftoO，a王ldthentoACC．Keywords：ACCoxidasc，＆r妇脚。弘k，CO，，Fc¨，in—11lblLor．K。vallle，p州ialp11nnc“o”Thcethylenep18ysanimpo^antr0Ieinmany8s—pectsDfp‰tgmw血anddevelopment，includingfnJitT1peningandsenescence(Abelesd“z，1992)．1’henn剞

6、stepofel}lvIenebiosvnt}Ie《siscaLalvzedbvthecnzvmeACCoxidasefformedyknownas出ee出ylene一凡rmingenzvme)．InsomccasesAOCoxidasealsop18ysanimportanttDIein血eregu【ation“ethyleneproduction，eginHlclon(Lasscrree￡口f．1996)，fomat0(BaⅡY以n2．1996)andRM，船zpoZ瑚旆Sm(vnezen出Ⅱf．1999)．OwinEto山e{actthat

7、ACCoxldase神tiv畸wascompk【clvlostuponhomogeni一盟lio”of￡1ssue．itwasonlv胡er山ediscovew￡ba￡i￡sm毗r。aclivi婶requircsascorbateandF一+thatpu打6一catlonandsubscⅡuentcharacterizationofthisenzvmehasbecomepossible(Ven．eridisandJohn199I)．ACCoxidascactivitvhasbcens七Lldiedinseveralcli—mactericfhit

8、ssuchasmelon(Smith“口f．1992)，avocado(Mccarv。yandChrist(，饪brsen1992)，a