溶血素的收集和溶血素单位的滴定

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1、实验四溶血素的收集和溶血素单位的滴定补体结合位点Fab段Fc段C1q结合位点被屏障结合抗原之前CH1CH2暴露的C1q结合位点结合抗原之后IgMCH3区，IgGCH2区Ⅰ原理Ag+AbComplementAg+Ab+C补体活化Ⅰ原理补体活化裂解Ⅰ原理SRBCMouseAnti-SRBCAb(hemolysin)免疫3times/3wsIgG+SRBCComplementHbHbHbSerumⅠ原理SRBC免疫小鼠2%SRBC补体(1:30)Ⅱ材料Test1Determinationofthetiterofhe

2、molysinⅢ方法1.收集血清摘取SRBC免疫小鼠眼球将血液收集至Ep管中4℃放置30min12000rpm离心10min取上层血清12345系列稀释1:5,1:10,1:20,1:40,1:80血清N.S0.4ml0.25ml0.25ml0.25ml0.25ml原液0.1ml0.25ml0.25ml0.25ml0.25ml稀释比例1:51:101:201:401:80mixmixmixmix123456N.S系列稀释血清①②③④Complement0.2ml0.2ml0.2ml0.2ml0.2ml0.2m

3、l彻底混匀37℃,30min2.溶血素单位的滴定0.2ml(Original)0.2ml(1:5)0.2ml(1:10)0.2ml(1:20)0.2ml(1:40)0.2ml(1:80)2%SRBC0.2ml0.2ml0.2ml0.2ml0.2ml0.2ml0.4ml0.4ml0.4ml0.4ml0.4ml0.4ml3.观察结果7----0.2ml0.6ml0.2mlⅣ结果Test1Determinationofthetiterofhemolysin完全溶血不完全溶血无溶血(对照)实验设计题目:经乙肝病毒表面

4、抗原（HBsAg）免疫的个体，如何定量检测相应特异性抗体的产生情况？内容：目的，方法，预期结果，讨论