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1、生态学报2010,30(4):1109—1116ActaEcologicaSinica短葶飞蓬总咖啡酸酯和灯盏乙素含量的空间变化3苏文华,张光飞,周鸿,闫海忠,周睿(云南大学生态学与地植物学研究所,云南昆明650091)摘要:依据嵌套分层设计原理制定野外调查取样方案,以药用资源植物短葶飞蓬不同产地93个自然种群310份植株样品的灯盏乙素和咖啡酸酯含量的嵌套方差分析,探讨次生代谢有效成分含量在产区间的空间变化。结果表明:短葶飞蓬有效成分含量存在空间差异,最低含量产区的总咖啡酸酯和灯盏乙素含量分别只是最高
2、含量产区的52.1%和41.8%,但不与产地间的空间距离远近完全一致;同一产地短葶飞蓬总咖啡酸酯和灯盏乙素含量有一定的年际差异,但高含量产地与低含量产地是相对稳定的;作为优质药材的基本产地单元,自然地理区划单元比行政区划单元更科学;短葶飞蓬总咖啡酸酯和灯盏乙素含量与植株含N量呈极显著负相关,符合“碳/氮营养平衡假说”的预测,但植株含N量的变化只能解释短葶飞蓬总咖啡酸酯和灯盏乙素含量不同产区间约30%的变异。植物药材有效成分含量的空间变化是药用植物地区间遗传变异和生长地环境生态因子变化共同作用的结果,用
3、自然地理区划单元为产地单元更客观。确定药材的“优质产地”可作为保障药材有效性的科学管理途径之一。关键词:短葶飞蓬;有效成分;含量;空间变化;碳/氮营养平衡假说SpatialvariabilityinthecontentofcaffeateandscutellarinofErigeronbreviscapus3SUWenhua,ZHANGGuangfei,ZHOUHong,YANHaizhong,ZHOURuiInstituteofEcologyandGeobotany,YunnanUniversity
4、,Kunming650091,ChinaAbstract:Spatialvariabilityincontentsofactiveconstituents,scutellarinandcaffeates,from310samplesin94naturalpopulationsofErigeronbreviscapus,amedicineherb,wasanalyzedinnestedANOVAwithintheproducingareasofthismedicineherb,basedonthesam
5、plingregimedesignedfollowingthenesteddesignprinciple.ResultsindicatedthattherewerevariationsincaffeatesandscutellarincontentofE.breviscapusgrowingindifferentproducingareas,averagecontentsofcaffeatesandscutellarininthelowestcontentproducingareaswere52.1%
6、and41.8%ofthatinthehighestcontentproducingarearespectively.Thedifferenceofcontentswasnotrelatedtothespatialdistancebetweenproducingareas.Thecontentofcaffeatesorscutellarinvariedannuallyinthesameproducingarea,butthelocationofthehighestorlowestcontentprod
7、ucingareadidnotchange.Thenaturaldivisionwasbettertodelimittheunitsofhighcontentproducingareathanadministrativdivision.Thecontentofcaffeatesorscutellarinwasnegativelycorrelatedwiththecontentofnitrogeninplantsgrowingindifferentareas,aspredictedascarbon/nu
8、trientbalancehypothesis.Thevariationinnitrogencontentexplainedabout30%ofthevariationinthecontentofcaffeatesorscutellarinofplantfromdifferentareas.Thespatialvariabilityinactiveconstituentcontentofmedicineherbswasresultedfromtheirg