IL18基因转染对小鼠卵巢癌OVHM细胞体内成瘤的影响

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1、IL18基因转染对小鼠卵巢癌OVHM细胞体内成瘤的影响【摘要】目的分析转染IL18基因后，对小鼠卵巢癌OVHM细胞体内成瘤的影响，初步探讨IL18基因转染治疗卵巢癌的应用价值。方法将逆转录病毒携带的小鼠IL18基因成功转染至OVHM（OVHM/IL18），以空载体转染的OVHM（OVHM/LXSN）和野生型（OVHM）作为对照，于裸鼠皮下分别接种细胞，观察各组种植瘤生长情况，计算成瘤率；HE染色及电镜分别观察病理组织切片的形态学改变。结果三组裸鼠的成瘤率相同，但OVHM/IL18组成瘤时间较晚，随瘤龄增长肿瘤生

2、长缓慢；瘤组织较小、界限清楚，血供不丰富，瘤细胞数量少、恶性程度较低，可见中性粒细胞、淋巴细胞浸润及细胞凋亡、组织坏死现象。OVHM组和OVHM/LXSN组的瘤组织较大、与周围组织界限不清，血管丰富，瘤细胞密集、恶性程度较高。结论IL18基因成功转染后，可能通过促进肿瘤细胞凋亡、抑制肿瘤血管形成及促进免疫浸润等机制，达到抑制肿瘤生长、降低卵巢癌恶性程度的抗瘤作用。【关键词】IL18；基因转染；OVHM；成瘤；裸鼠基金项目：河北省卫生厅科研课题基金资助项目（04234）EffectofIL18GeneTransfecti

3、onontheTumorigenesisinVivoofMouseOvarianCancerCellLineOVHMCUICheng*,HAOShuweiBAIXiaojia,etal.*BethuneMilitaryMedicalCollege，Shijiazhuang050081，China【Abstract】ObjectiveToinvestigatetheeffectofIL18genetransfectionontumorigenesisofmouseovariancancercelllineOVHM,andto

4、discussthepreliminaryvalueofIL18genetransfectionintreatmentofovariancancer.MethodsOVHMwastransfectedwithretroviruswithIL18genenamedasOVHM/IL18,andthewildOVHMandOVHMtransfectedwithvectorwithoutIL18gene(OVHM/LXSN)wereenrolledascontrol.Thenudemicewereinoculatedwit

5、hdifferentcellssubcutaneously,thenthegrowthoftransplantedtumorwasobserved,theratiosoftumorigenesiswerecalculatedandthemorphologyoftumorcellintissuewasanalyzedbyHEstainingandelectronmicroscope.ResultsAfterinoculatedrespectively,theratiosoftumorigenesisweresimilarint

6、hreemicegroups.ButinOVHM/IL18group,thelatentperiodoftumorigenesiswaslongerthanthatofothertwogroups,theslowerspeedalongwithtumorgrowthwasobserved;thetumortissuewassmallerandhadalimitedboundary,thenumberofbloodvesselandtumorcellswerefewer,andthemalignanceoftumorcell

7、swaslower;theapoptosis,coagulativenecrosisandtheinfiltrationofneutrophilicgranulocytesandlymphocyteswereobserved.Meanwhile,thetypicalmorphologyofapoptosiswasshowedbyelectronmicroscope.However,thetumortissuesofOVHMandOVHM/LXSNgroupswerelargerandhadunclearboundary,th

8、enumberofbloodvesselandtumorcellsweremore,themalignanceoftumorcellswashigher.ConclusionAlthoughIL18genetransfectionsuccessfullycannotdisplaycyto