川产道地药材黄柏HPLC指纹图谱质量标准研究

《川产道地药材黄柏HPLC指纹图谱质量标准研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、西南交通大学硕士学位论文川产道地药材黄柏HPLC指纹图谱质量标准研究姓名：杜雪申请学位级别：硕士专业：生物化学与分子生物学指导教师：张小荣20071101西南交通大学硕士研究生学位论文第fl页本研究对川黄柏药材指纹图谱进行的初步探索性研究可为相关研究提供一定参考，但RP-DAD色谱指纹图谱只能检测有紫外吸收的成分，还有很多无紫外吸收的成分不能在指纹图谱中反映出来。由于小檗碱含量较高，其它成份的峰高相对较低，造成含量较少成分细微特征的丢失。对该药材的化学成分及质量控制研究仍有待深入。关键词：川黄柏：化学成分；铁屎米酮．6：黄柏酮；HPLC：指纹图谱西南交通

2、大学硕士研究生学位论文第1ll页Abstract硼埒drybarkofRutacoaeplantPhellodendronchinenseSchneid．iscalledHuangbo．It'salsoknownasChuanhuangbousually．RisoneofthemostconnnonlyusedTraditionalChineseMedicineswithitsverywideclinicalapplicationsineFmainatingheatanddampness，purgingsthcnicfire，reducingasthen

3、ia-hcatandbonesteaming,relievingtoxinforpyocutancousdiseases，andetc．TheChinesePharmacopoeiain2005versionseparatedHuangbofromGuanhuangbothatderivedfromPhellodendronamu棚eRupr．foritsindividualfunctionsasallherbalmedicine．Thea叫TemqualitycontroloftheherbHuangboanditspreparationmanufac

4、turingisbasedonthequalitativeandquantitativeanalysisofberberine．Thoughsomeknownchemicalconstituentssuchaspalmatineandjatrorrhizinehavebeenrecoveredandmayassistthequalitycontrolstudy,itisstilllackofevidencetocharacterizeHuangbo’soverallqualityproperties．InordertouseHuangboasamedic

5、meappropriatelyanddevelopitsqualitycontrol，thispaperdidaprelmamrystudyonitschemicalcompositionsandfingerprints．SeparationandpurificationwereperformedbythesilicagelColumnChromatography．Twocompoundswe∞isolatedandidentifiedasCanthin-6-one①andObacunone(珂．It'sthefirsttimetogetCanthin-

6、6-one(DfromthebarkofPhellodendronch加e，l卵Schneid．ThepreliminaryexperimentwasperformedbyultrasonicextractionandUVdiodearraydetector．TwelvesamplesofHuangbopurchasedindifferentareasweretestedandprepared丽thl％aceticacid-methan01．SeparationWasperformedOilaHypersilBDSCIscolumnforthelarge

7、pHrangetolerance．ThemobilephaseWascomposedby0．3％triethylaminephosphate-waterandacetonitriletodothegradientelution(0-15rain，acetonitrile15％也5％：西南交通大学硕士研究生学位论文第1V页15—35min,acetonitrile25％--40％；35～40min,acetonitrile40％．-90％；椭Omin．acetonitrile90％～100％)attheflowrateofo．8mVmin．The珂ecti

8、onvolume’was5ttl．TheUVdetectionwavelengt