大鼠成年胰腺与胚胎185天胰腺蛋白表达谱初探

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1、南京医科大学硕士学位论文大鼠成年胰腺与胚胎18.5天胰腺蛋白表达谱初探姓名：程志祥申请学位级别：硕士专业：生物化学指导教师：德伟20030401舞臻莲群太攀壤圭攀髓诹絮太鼠成年胰缘与麒贻18。5天髓腺萤癌表达谱襁撂磺士研霓生：糕志祥导澄：德伟南京缀稃大学生耱化学及分子生物学系，南京，210029攘要拳磷究爨残双惫凝'

2、i乏遗溶技拳分离SD走鼠成年麓臁纭缀和胚鼹i8。5一_=：y天胰腺组织总赞毒，锻兼和考染显包，荻谨了分辨率争譬复挂均较好媳双内电泳潮谱。煺ImageMaster(Pharmacia

3、公翅涸豫袄件分橱，发现1000±50个蚤爨震意在成莘胰稼中捡溺到表达，800_--'250个蛋霸质点在秘黯i8．5天骥豫申^硷溅到表达，莫孛8令蛋蠢痿点摩箴年液艨组织中含量降诋，20个蛋白震点含螫增加。。另盈少有4个蛋巍质点在成年胰腺纽缴缺失，14令蛋白艘点成年胰骧缎织特宥。鼠审随热选择一姥差异嚣白袭点，避籽基震藕动激竞簿啜一电离飞行对阚质-滋-(MALDI-TOF。MS)淤I定其虢肉酶解后簿黢震糖纹蓬谱，蹙Mascot软孛}壹馋Swiss，port数括毒，劫爹鏊定为～鎏与生长发育、耪瘊4-ji

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5、簿飚蘑予、信号转导等有炎蜒赞魉屡，如ERP60、trypsinogen、proelastase、lipase等+这种鹫巍震甄戆差异分折宥劝于麟究蕊腺发肖相关蛋白质及分子标记耪，溪臻胰腺盼发言抵制。为糖尿癌盼预跨、诊躜、治疗等挺绥蓊掌论据。．r二一一芰荧键词】太鼠；魈艟；姨躁；双奄电泳；毽像分据；疑或鼹纹澄港鬻亵医科丈攀磺士学鲢谗文PreliminaryAnalysisofRatPancreasProteomebetweenAdultandEmbryonic18．5Departmento

6、fBiochemistry&MolecularBiologyNanjingMedicalUniversity,Nanjing，10029，ChinaPostgraduate：ChengZhixiangTutorPro您ssor：De融iAbstractToprovideevidence南}illustratingthemolecularmechanismofpancreasdevelopment，proteinswereextractedfromratpancreastissueatvadous

7、stages，separatedbyhighresolutiontwo—dimensionalelectrophoresis(2．DE)，andthegood2DEpatternincludingresolutionandreproducibilitywasobtained，Aftersilverstaining，the2DEimageanalysisbyImagemaster2DEsoftwarehaddetected800±50spotsinE18。5pancreasmapand1000±5

8、0spotsinadultpancreasmap，ofwhich8spotsincreasedand20spotsdecreasedinquantity．Furthermore，4spotsweremissingandt4flewemergedinadultratpancreascomparedwithE18。5ratpancreas。Forproteinidentification，proteinsspotsofinterestonthegelssminedwithcolloidalCooma

9、ssieBrilliantBlueG-250we}eexcised，digestedin⋯gelwithtrypsin，andanalyzedbypeptidemassfingerprinting(PMF)withmatrixassi或edlaserdesorption／ionization-massspectrometry(MALDt*MS)．ERP60、trypsinogen，proelastase，lipaseandsoonwereidentified。ThiswillenableItSt

10、opresentanoverviewoftheproteins々南京医科大学硕士学位论文expressedinratpancreastissuesandlaysthebasisforsubsequentcomparativeproteomeanalysisstudieswithpancreasdevelopment．[Keywords】rat；pancreas；embryo；two—dimensionalgelelectmphoresis(2DE)；peptidemassfingerprinti