大承气汤对大鼠盲肠结扎穿孔致多器官功能障碍的保护作用

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1、广西医科大学硕士学位论文大承气汤对大鼠盲肠结扎穿孔致多器官功能障碍的保护作用姓名：陈文丽申请学位级别：硕士专业：病理生理学指导教师：程建祥2003.6.1大承气汤对大鼠盲肠结扎并穿子L致多器官功能障碍的保护作用摘要目的：为了探讨大鼠盲肠结扎并穿孔引起肠外多器官功能障碍的作用及其机理，并观察大承气汤对多器官功能障碍的保护作用。方法：实验采用无菌丝线结扎盲肠远端1／2处，并用无菌九号针头在已结扎的盲肠远端中部贯通穿刺，造成腹腔感染，建立损伤模型。造模后即以大承气汤灌胃。结果：盲肠结扎并穿孔术后36h肝肾功能的生化指标、肺湿／干比值、肝、肺、肾组织中的Id

2、DA明显升高，SOD活性下降，16h外周血TNF-Q浓度增加，而大承气汤可缓解CLP对肠外器官功能障碍，减少MDA的生成，增强SOD的活性，抑制TNF-Q的生成。结论：盲肠结扎并穿孔可导致多器官功能障碍，可能与炎症反应失控过度释放TNF-Q等炎症介质和过多氧自由基的毒性作用有关。大承气汤对CLP引起的多器官功能障碍有多方面的保护作用。关键词：大承气汤：多器官功能障碍综合症；CLP√v／)

3、atsABSTRCTobjective：toexploreprotectiveeffectsofDCQDonmultipleorgandysfunctionduringcecalligationpunctureinratsandtostudythemechanismbywhichthemultipleorganwasdamaged．Method：MODSwasinducedbycecalligationandpunctured(CLP)．Thececumwasexteriorized．Tllledistalendofthececum(about50％

4、ofthetotalcecallength)wasligatedwithsterile4-0silkandpuncturedtwicewithasterile0．9mmneedleinthecenteroftheligatedportion．Afterclosureofthewound，DCQDwaspoureddownintothestomachofratsintheexperiments．Results：aftercecalligatedpunctureinjury，thebiochemistryindexsofliverandkidney、th

5、elungwet／dryweigh、thelevelofMDAwasgraduallyincreasedafterCLP36hwhiletheactivitiesofSODwasgraduallydecreasedafterCLP36h；TNF-CIconcentrationsrapidlyincreaseafterCLP16h．DCQDcandecreasethelevelofMDA、thelungwet／dryweighandthebiochemistryindexswhileimprovedtheactivitiesofSOD．Conclusi

6、on：CLPcanleadtoMODS．ThemechanismmaybeinvolredinpoisiouseffectsofabnormalmediatorsuchasTNF-Qresultingfromoverwhelminginflammationandexcessiverestionoxygenspecies．DCQDcannotedlyimprovedthefunctionoforgansandhaveapparentseffectsontheinfectiousmodels．Keyword：DachengqiTang；Multipleo

7、rgandysfunctionsyndrome；CLP’2CLP(cecal1igationand主要缩略语puncture)盲肠结扎并穿孔DCQD(Dachengqidecotion)__大承气汤SIRS(SystemicInflammatoryResponsesyndrome)一全身炎症反应综合症MODS(MuItipleOrganDisfunctionSyndrome)～多器官障碍综合症SOD(SuperoxideDismutase)一超氧化物歧化酶MDA(Malondialdehyd刨一丙二醛PAF(PlateletActivatingFac

8、tor)一血小板活化因子PMN(PeripheralBloodMononuclearNeutrophil)一