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时间:2018-12-05
《临床医学毕业论文异氟醚后处理对盲肠结扎穿孔大鼠急性肺损伤的保护作用》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、异氟醚后处理对盲肠结扎穿孔大鼠急性肺损伤的保护作用姓名:2014年6月25日异氟醚后处理对盲肠结扎穿孔大鼠急性肺损伤的保护作用作者:徐文莉1,何俊1,曾因明1,庞庆丰【摘要】目的观察异觀醚(isoflurane,ISO)后处理对盲肠结扎穿孔术(cecalligationandpuncture,CLP)后大鼠急性肺损伤(acutelunginjury,AL1)的保护作用。方法32只SD雄性大鼠随机分为4组(每组n=8):正常组(N组),未行任何处理;假手术组(Sham组),单纯行开腹关腹术;盲肠结扎穿孔术组(
2、CLP组),行CLP建立大鼠ALI模型;异氟醚组(ISO组),建立CLP模型后4h腹腔注射液态异氟醚2ml/kg。术后12h各组动物腹主动脉放血处死,右肺进行病理切片和肺湿/干比(W/D)测定,组织匀浆测超氧化物歧化酶(SOD)、丙二醛(MDA)和髓过氧化物酶(MPO)的含量;左肺行支气管肺泡灌洗,取上清液测总蛋白、肿瘤坏死因子ot(TNF-a)和白细胞介素10(IL-10)的含量。结果肺组织病理学检测结果显示CLP组大鼠肺组织损伤严重,ISO组肺组织损伤较轻。与N组比较,CLP组和ISO组屮SOD含量降低
3、(P0.01),其余检测指标均增加(P0.01)。与CLP组比较,ISO组SOD和IL-10含量较高(P0.01);其余检测指标均低于CLP组(P0.05或P0.01)。结论在木实验条件下,大鼠腹腔注射液态ISO可发挥抗炎、抗氧化作用,对CLP所致大鼠ALI有部分保护作用。【关键词】急性肺损伤;肺保护;异氟醚;后处理;盲肠结扎穿孔Abstract:ObjectiveToinvestigatetheprotectiveeffectsofisoflurane(ISO)post-treatmentonacutel
4、unginjury(ALI)inducedbycecalligationandpuncture(CLP)inrats.Methods32maleSprague-Dawley(SD)ratswererandomlydividedinto4groups(n=8each):normal(N)group,withnotreatment;Sham-operatedcontrol(Sham)group,withmereincisionandclosureoftheabdomen;cecalligationandpunc
5、ture(CLP)group,establishedasratmodelofinducedALI;andisoflurane(ISO)group,withintraperitonealinjectionof2ml/kgISO4hafterCLP.Theanimalsweresacrificedbyexsanguinationviatheabdominalaorta12hafterCLP.Therightlungswereremovedforpathologicaldissection;determinati
6、onoflungwet-to-dryweightratio(W/D);determinationofthecontentofsuperoxidedismutase(SOD),malondialdehyde(MDA)andmyeloperoxidase(MPO)inthepulmonaryhomogenate.Followinglavagesoftheleftlungs,thesupernatantfromthebronchoalveolarlavagefluid(BALF)wasdeterminedfort
7、hetotalproteinlevel,thecontentoftumornecrosisfactor-a(TNF-a)andinterleukin-10(IL-10).ResultsThepathologicalresultsshowedthatratsinCLPgrouphadseriouslunginjury,whiletheratsinISOgrouphadslightinjury.InbothCLPandISOgroups,theSODcontentdecreased(P0.01)andtheot
8、herparametersincreased(P0.01),ascomparedtotheNgroup.TheSODandIL-10contentswerehigherinISOgroupthaninCLPgroup(P0.01).ThecontentsofMDA,MPOandTNF-aandW/DwerelowerinISOgroupthaninCLPgroup(P0.05orP0.01).Conclusion
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