羟苯磺酸钙对慢性肾功能不全大鼠TGFβ1、BMP7表达的影响

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1、·中文论著摘要·羟苯磺酸钙对慢性肾功能不全大鼠TGF一131、BMP一7表达的影响目的肾脏纤维化是各种病因肾脏疾病导致慢性肾功能不全的共同通路，延缓甚至逆转肾脏纤维化，减少肾功不全的发生与进展成为肾脏病工作者的目标，但目前临床仍无确切方法可以做到。本实验通过观察羟苯磺酸钙对慢性肾功能不全大鼠TGF．13l和BMP．7的影响，从致纤维化和抗纤维化两个角度证实羟苯磺酸钙改善肾脏纤维化的作用。方法SD大鼠32只，对照组8只、模型组12只、治疗组12只，模型组和治疗组采用手术切除左肾加两次尾静脉注射阿霉素法制成慢性肾功能不全模型、对照组行假手术，并注

2、射等量生理盐水；治疗组第5周开始予羟苯磺酸钙100mg／kg·d灌胃，对照组和模型组等量生理盐水灌胃，分别于实验第6周末与第8周末处死对照组4只、模型组及治疗组各6只大鼠，摘取其肾脏、取材、固定、石蜡包埋、切片、染色、光镜下观察肾脏病理改变，并进行免疫组化染色，比较对照组、模型组、治疗组之间的差异。结果病理改变：HE、Masson染色见模型组肾小球系膜细胞增生，系膜基质增多，球囊粘连，局灶节段性硬化、肾小管上皮细胞颗粒变性，肾小管上皮细胞脱落、肾小管扩张、肾间质纤维化程度重，治疗组用药2周及4周与模型组比较病变减轻；免疫组化显示TGF．pl在

3、模型组肾小球、肾小管大量表达，治疗组用药2周及4周与模型组比较表达明显减少(P

4、eKidneyfibrosisincludingglomerularsclerosisandrenalinterstitialfibrosis，isakeyeventleadtorenalfailuredespiteofdifferentdisease，theunequivocalmechanismofwhichremainsunclear．DelayperhapshaltkidneyfibrosisprovetobeOurhope．Howeverwehavenotfindaeffectwaytoimplementit．Ofnotekidne

5、yfibrosisoccureswhenlostthebalanceofthefactoresleadtoandpreventfibrosis，duringthiscourselotsofcellfactorsattended．InOurclincleworkwefindCalciumdobesilatecutdownserumcreatinine．wetreatCRFrat、^rithCalciumdobesilateandstudyTGF一131andBMP一7expressionintheanimal．MethodsThirty—two

6、ratsarerandomlydividedintosham(n=8)，model(n=12)andcalciumdobesilategroup(n=12)Firstexcepttheshamoperationgroup，underwentadoptoperation,atthelweekaffteroperationmodelgropandcalciumdobesilategroupintravenousinjectionAdriamgcinfromtalevein4mg／kg，withshamgroupintravenousinjecti

7、onsaline，atthe4weekmodelgropandcalciumdobesilategroupintravenousinjectionAdriamgcinfromtalevein2mg／kg，withshamgroupintravenousinjectionsaline，CRFmodelwereestablished．Duringthe5tothe8weekcalciumdobesilatewereirrigatedintothestomachstothecalciumdobesilategroup．Atthe6weekandth

8、e8weekoftheexperimentsacrificedrats，removalkidneywerefixed，observewithlightmicrosc