资源描述：
《水稻泛素结合酶基因家族的生物信息学与表达分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、中国水稻科学(ChinJRiceSci),２０１６,３０(３):２２３－２３１http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１７７２２３水稻泛素结合酶基因家族的生物信息学与表达分析刘鑫张恒阚虎飞周立帅黄昊宋林林翟焕趁张君鲁国东∗(福建农林大学功能基因组学研究中心,福州３５０００２;∗通讯联系人,EＧmail:gdlufafu＠１６３．com)BioinformaticandExpressionAnalysisofRiceUbiquitinＧco
2、njugatingEnzymeGeneFamilyLIUXin,ZHANGHeng,KANHuＧfei,ZHOULiＧshuai,HUANGHao,SONGLinＧlin,ZHAIHuanＧchen,ZHANGJun,LUGuoＧdong∗(TheFunctionalGenomicsCenter,FujianAgricultureandForestryUniversity,Fuzhou３５０００２,China;∗Correspondingauthor,EＧmail:gdlufafu＠１６３c．om)L
3、IUXin,ZHANGHeng,KANHufei,etal．BioinformaticandexpressionanalysisofriceubiquitinＧconjugatingenzymegenefamily．ChinJRiceSci,２０１６,３０(３):２２３Ｇ２３１．Abstract:Theubiquitin/proteasomesystemplaysanimportantroleinplantgrowthanddevelopment,morphogenesisanddiseaseresi
4、stance．Recentstudieshaveshownthatsomepathogenscanmimicthehostplantubiquitin/proteasomesystemcomponentstoachievetheirownpurposes．UbiquitinＧconjugatingenzymeisthesecondenzymeintheubiquitinationprocessandisindispensablefortheplantubiquitin/proteasomesystem
5、．Previousstudiesshowedthatthereare４８predictedubiquitinＧconjugatingenzymegenesinricegenome．InordertopreliminarilyelucidatethefunctionsofriceubiquitinＧconjugatingenzymegenesinplantdiseaseresistance,bioinformatic,RNAＧseqandqRTＧPCRmethodswereusedtoanalyzech
6、aracteristicsandexpressionpatternsofriceubiquitinＧconjugatingenzymegenefamily．Phylogenetictreeanalysesindicatethatthe４８riceubiquitinＧconjugatingenzymegenescanbedividedinto３groups,７subＧgroupsintotal．ProteindomainanalysisshowedthatubiquitinＧconjugatingenz
7、ymegenesmainlyconsistofabigubiquitinＧconjugatingenzymecatalyticdomain．ExpressionanalysisinsilicosuggestedthatmostofthericeubiquitinＧconjugatingenzymescanbeinducedbyblastfungusinfection．PlantcisＧactingelementsanalysisindicatedthatfourpathogenresistanceci
8、sＧactingelementsandonehypersensitivityreactioncisＧactingelementhavehighdistributioninthepromoterregionofthe４８riceubiquitinＧconjugatingenzymegenes．RNAＧseqdatafromcompatibleandincompatiblemonogenicriceafterriceblastfungusinfections
