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1、中国水稻科学(ChinJRiceSci),２０１６,３０(１):１７－２６http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５０９２１７云南地方稻种抗稻瘟病基因PiＧd３序列变异分析杨奕孙一丁马继琼王炎炎许明辉∗(云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创制重点实验室,昆明６５０２２３;∗通讯联系人,EＧmail:xuminhui＠sohu．com)SequenceVariationofPiＧd３forRiceBlastResistanceinYu
2、nnanRiceLandraceYANGYi,SUNYiＧding,MAJiＧqiong,WANGYanＧyan,XUMingＧhui∗(InstituteofBiotechnologyandGeneticResources/KeyLaboratoryofBiotechnologyResearchofYunnanProvince/KeyLaboratoryofSouthwesternCropGeneResourcesandGermplasmInnovationofMinistryofAgriculture,YunnanAcademyofAgricultura
3、lSciＧences,Kunming６５０２２３,China;∗Correspondingauthor,EＧmail:xuminhui＠sohuc．om)YANGYi,SUNYiding,MAJiqiong,etal．SequencevariationofPiＧd３forriceblastresistanceinYunnanricelandＧrace．ChinJRiceSci,２０１６,３０(１):１７Ｇ２６．Abstract:ThesequencevariationsofPiＧd３forriceblastresistancein８０Yunnanricela
4、ndraceswereanalyzedbyreseＧDiguquencingopenreadingframe(ORF)．ComparedwithPiＧd３ORF,atotalof３９nucleotidevariationswerefoundin２７７５ＧbpORFsof８０riceaccessionswiththeaveragemutationrateof１．４１％．TheWTBXPiＧd３ORFof８０riceaccesＧsionswasdividedinto３７haplotypesbasedonthenucleotidevariations．H８(２８．
5、８％),H４(１１．３％)andH２３(５．０％)arehighＧfrequencytypes,whileothertypesoccurredatlowfrequency．TheresultsshowedthattheWTBXPiＧd３inYunnanricelandraceswasrichingeneticvariationsandhaplotypes,butlowfrequency．Atotalof２８aminoacidsvariationswerefoundin３３translationproteinsof３７haplotypes．１８haplotyp
6、eswith３２ricelandracesincluding１２indicaaccessionspossessdethePiＧd３pseudogenes．Thefrequencyofpseudogenewashigh．ItwaspossibleduetothatthePiＧd３pseudoＧgenepermeatedtoindica．Terminatorswerenewlyfoundedatposition３２and４６７ofproteinexceptthepublishedtermiＧnatoratposition７３７．PiＧd３differedinbo
7、thtypeandfrequencyofhaplotypesbetweenthesubspeciesofindicaandjaＧponica,theecotypesoflowlandanduplandrice,noＧwaxyandwaxyrice．ItindicatedthatgeneticdifferentiationocＧcurredbetweensubspeciesorecotypes．Geographicaldistributionshowedthat３７haplotypesspreadoveralargeareawhileconcentratedi
8、nsmallareas．Puer,BannaandL
