精液对子宫内膜蜕膜化及胚胎发育的影响

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1、精液对子宫内膜蜕膜亿及胚胎发育的影晌硕士研究生：朱丽丽指导教师：石红教授专业名称：妇产科学摘要目的：探讨精液成分对子宫内膜蜕膜化及胚胎发育的影响。方法：i．分离育龄麓妇女增麓晚弱及分泌期子宫雨貘缁脆，翻入雌二醇(ED、孕酮(P)使其蜕膜化，分别加入人精子及精浆共培养，观察蜕旗化魏闻质细胞生长祷况并溺定培养液中泌乳素PRL含璧。2．内膜培养8天聪，加入小鼠2一细胞期胚胎继续培养，观察胚胎的发育情况及内膜细胞生长情况。3．建立假孕鼠模型，观察精液在胚胎移植中的{乍用。缩果：1．加入精浆的子宫内膜间质细胞蜕膜化程度明显优于精子龌积对照组。糖浆缀分泌PRL鲶爨与糖予组稷

2、对照缝翱毙有显著瞧差异(p<0．05)，而精子组和对照组无明显蒺异。2．精浆能促进八细胞以后熬簇艟在予富海貘缨魏上的癸裂、囊凝形成、孵化、嚣辩翻扩震往生长(p<0．05)。3．胚胎移植过程中精子对植入率没有影响。绪论：l精浆对子富内膜闻凄细胞蜕膜耽有髓显健递作用，能够促进PRL的分泌。2。糟浆的存在可以促进胚胎在子宫内膜细胞共培养体系中的发育。3．以输卵管缩扎和假孕鼠为受体的胚胎移植着床率无明显差异提示了糖浆与糖液对囊胚着床终划可能稠同。关键谰：予富农膜蜕膜诧PRL糌浆胚胎发寒Theeffectsofsemenonhumanendometrialstromace

3、llsdecidulizationandembryodevelopmentPostgraduate：ZhuLiliSupervisor：ShiHongMajor：ObstetricsandGynecologyABSTRACTobjeetive：toinvestigatetherolesofsemeninregulationofendometrialstromacellsdecidualizationandembryodevelopmentMethods：1．Stromacellsderivedfromhumanendometriumduringthelatepr

4、oliferatephaseorsecretivephaseweretreatedwithestradiol、progesteronetoinducedecidualization．Spermatozaandseminalplasma，isolatedfromnormalmenwereaddedtothestromacells．Thestromacellsmorphologicchangesandprolactin(PRL)productioninthemediawereexaminedandcompared。2．The2-cellmouseembryowere

5、addedtothestromacellsaftercultured8days．The‘embryogrowthrateandblastocystformationrate、hatching、adhereing、outspreadingofmouseembryoandthedevelopmentofstromacellswereobservedunderthemicroscope．3．Inordertoobservetheeffectofspermatozoaonimplantationrate，theblastocystsweretransferredtoda

6、y3fakepregnantfemalemiceandcontrolgroup．Results：1．Seminalplasma(SP)significantlyincreasedthedegreeofthestromacellsdecidualizationcomparedwithspermatozaandthecontrolgroup．PRLsecretionofCO—culturedwithSPwassignificantlyhigherthantheothertwogroups(p

7、ryogrowthrate(after8-cell)andblastocystformationrate、hatching、adhering、outspreadingontheendometrialstromacells(p<0．05)．3．Theimplantationrateoftwogroupswerenodifferenceispermatozoahaveno·2‘ef艳ct。eonc

8、usion：1．SPea熊contributetothestromacellsdecidualizationandimprovePRLsecretion．2．SPcarl

9、promotetheem