EGFL7基因沉默对裸鼠乳腺癌血管生成的影响

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1、万方数据·476·EGFL7基因沉默对裸鼠乳腺癌血管生成的影响刘志勇欧阳忠邹小明【摘要】目的探讨类表皮生长因子域7(EGFL7)基因沉默对乳腺癌裸鼠模型瘤内血管生成的影响。方法构建EGFL7短发夹状RNA表达质粒并转染SGC-7901细胞(pshEGFL7组)，以空质粒转染为对照组。观察两组皮下种植瘤生长曲线及体积。免疫组织化学法检测抗．CD34、血管内皮生长因子(VEGF)、血小板反应蛋白(TSPI)表达；RT-PCR检测MMP-2和TIMP2表达。结果pshEGFL7组种植瘤体积为(1．86±0．65)cm3，微血管密度(MVD)为20．84

2、士6．38-，分级为1—2级，对照组体积为(4．864-1．15)cm3，MVD为39．48士9．01，分级为3～4级，两组种植瘤体积、MVD和分级的差异有统计学意义，P值均<0．05。EGFL7组TSPI蛋白阳性表达，而VEGF蛋白为弱阳性或阴性表达，MMP-2mRNA表达下调，TIMP2mRNA表达上调，与对照组比较差异有统计学意义，P值均<0．0l。结论EGFL7基因沉默可降低乳腺癌种植瘤血管生成，与调节MMP-2／TIMP2表达和影响VEGF／TSPI平衡有关。【关键词】短发夹状RNA；乳腺癌；血管生成；类表皮生长因子域7；裸鼠Effec

3、tofRNAinterferencetargetingatEGFL7onangiogenesisofbreastcarcinomainnudemiceLIUZhi-yong。OUYang-zhong，ZOUXiao-ming．TheFirstAffiliatedHospitdofC,anNanMedicalUniversity，Can-zhou341000，ChinaCorrespondingauthor：OUYang·zhong，E·mail：oyzhyl@163．com【Abst憎n】ObjectiveToinvestigatetheeffe

4、ctofshorthairpinRNA(shRNA)targetingatepidermalgrowthfactor-1ikedomain7(EG凡7)geneonangiogenesisofbreastcarcinomaanditsmechanisminnudemice．MethodsshRNAtargetingatEGFL7geneWa$constructedandtransfectedintoSGC-7901cells(pshEGFL7group)，meanwhile，thecellstransfectedwithvectorplasmid

5、swere∞acontrolgroup．Positivecloneswereselectedandthetransplantedtumoranimalmodelsconstructedinnudemice，andthegrowthandvolumeofmmor葛wereobserved．After8weeks．EGFL7mRNAandproteinintransplantedtumortissuesweredetected，andgraded．Moreover，Anti—CD34，VEGFandTSPlwerestainedbytheimmuno

6、—chemistrymethod，andMMP-2andTIMP2mRNAweredetectedbyRT．PCR．ResultsEGFL7mRNAwasdownregulatedsignifi-cantlyinthepshEGFL7group．InthepshEGFL7group，thetumorvolumewas(1．864-0．65)cm’，MVDWflg20．84士6．38；whileinthecontrolgroup，tumorvolumewas(4．864-1．15)cm3，MVDWas39．48±9．01，IntheEGFL7gro

7、up．TSPIproteinpresentedpositive，andVEGFproteinpresentedweaklypositiveornegative．TheexpressionofMMP-2mRNAdecreased，T[MP2mRNAincreasedinthepshEGFL7group，andthereweresignificantdifferencescomparedwiththecontrolgroup。P<0．01．ConclusionRNAinterferencetargetingatEGFL7geneCanbalanceT

8、SPl／VEGF，throug}lregulatingtheexpressionofMMP-2／TIMP2toimpairangioge